摘要
利用AFLP分析技术，从64对PstI／MseI引物中筛选出9对多态性引物组合，对来源于中国广东、福建、台湾的42个墨兰品种进行了遗传多样性和亲缘关系分析，共扩增出1384条带，其中多态性带1333条，多态性率96．3％。平均每对引物扩增带数154条、多态性带数148条。所有的品种均可区分，其中39个品种具特征带或缺失带。墨兰品种间的遗传多样性丰富，品种间的Dice相似系数0．4222～0．8245，平均相似系数0．6112。“达摩”系列芽变叶艺品种间的遗传多样性显著降低，多态性率76．6％，平均相似系数0．7256。NTSYS2．10聚类分析表明同一产地来源的品种基本上可聚在一起，反映出了品种间的亲缘关系。
AFLP analytic method was tested in the forty-two cultivars of Cymbidium sinense cuhivars, originated from Guangdong, Fujian, and Taiwan provinces of China. Genomic DNA was extracted from the green leaves of Cymbidium sinense cultivars by the method of CTAB 9 pairs reproducible primers were selected from 64 pairs of primers. A total of 1 384 bands were observed, of which 1 333 bands （96. 3% ） were polymorphic. Each pair of primers produced 154 bands following amplification. The AFLP markers indicated that all cultivars were of different cuhivar. 39 cuhivars had their specific bands or deleted bands. The Dice coefficients amongst the cuhivars ranged from 0. 422 2 to 0. 824 5, with an average value at 0. 611 2. The polymorphic ratio of C. sinense ‘Da Mo＇ with sport mutations is 76.6%. The Dice coefficients amongst the cuhivars ranged from 0. 616 4 to 0. 824 5, with an average value at 0. 725 6. According to the dendrogram using unweighted pair group method using arithmetic averages （UPGMA）, the cuhivars were grouped into three main clusters. Cuhivars with the same origin could be clustered together.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第3期69-73,共5页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
广东省科技攻关重大专项资助项目(2003A20104012006A2010401)
广东省农科院院长基金资助项目(2001-基金-24)
关键词
墨兰
兰科
遗传多样性
AFLP
芽变
叶艺
Cymbidium sinense
Orchidaceae
genetic diversity
AFLP
spot mutation
foliage variegation
