摘要
目的:研究α-硫辛酸(alpha-lipoic acid,α-LA)对高脂血症新西兰兔血管内皮细胞氧化损伤的保护作用。方法:24只新西兰大白兔根据不同喂养方案分为正常组、造模组、硫辛酸组,然后分别检测各组兔的甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、一氧化氮(nitric oxide,NO)水平。结果:24只兔全部进入结果分析。造模组的血清TC、TG、LDL-C水平均显著高于正常组(P<0.01),HDL-C显著低于正常组(P<0.01)。硫辛酸组的血清TC、TG、LDL-C水平均显著低于造模组(P<0.01),HDL-C无明显变化(P>0.05)。造模组的SOD和NO含量显著低于正常组(P<0.01),而MDA含量显著高于正常组(P<0.01)。硫辛酸组的SOD和NO含量高于造模组(P<0.01),而MDA含量显著低于造模组(P<0.01)。结论:α-LA具有预防动脉粥样硬化的作用,其机制可能与清除超氧阴离子,降低氧化应激对血管内皮细胞的氧化损伤有关。
Objective To ascertain the protective effects of alpha-lipoic acid （α-LA） on vascular endothelial cells in New Zealand rabbits with hyperlipidemia. Method 24 New Zealand rabbits were randomly assigned to continuously receive normal diet （normal control group）, high fat diet （model group）, or high fat diet ＋ 300 mg/d α-LA （α-LA group） for 12 weeks. At the end of the experiment, serum levels of triglyceride （TG）, total cholesterol （TC）, high density lipoprotein cholesterol （HDL-C）, low density lipoprotein cholesterol （LDL-C）, malondialdehyde （MDA）, superoxide dismutase （SOD）, and nitric oxide （NO） were detected. Results As compared to normal control group and α-LA group, serum levels of TC, TG, LDL-C, and MDA were significantly higher （P 〈 0.01 ）, and levels of SOD and NO were significantly lower （P 〈 0.01 ） in model group. Level of HDL-C in model group was significantly lower than that in normal control group （P 〈 0.01 ）. Conclusion ot-LA can effectively inhibit the development of atherosclerosis by cleaning the superoxide anion and decreasing the oxidative injury of vascular endothelium.
出处
《实用医学杂志》
CAS
北大核心
2009年第10期1559-1561,共3页
The Journal of Practical Medicine
基金
广东省科技计划项目(编号:2008B030301153)
