摘要
目的观察血小板衍生生长因子（PDGF）A、B及其α、β受体在大鼠野百合碱肺高压模型中的表达，探讨PDGF在肺血管重构中的作用。方法SD大鼠24只，体质量250—300g，随机分为野百合碱注射组（MCT，12只）和对照组（Con，12只）。MCT组给与野百合碱60mg／kg单次皮下注射。喂养6周后，使用逆转录-聚合酶链反应（RT-PCR）观察肺组织PDGF—A、PDGF—B及其仪、B受体mRNA转录量的变化。结果MCT组肺组织中PDGF—A、PDGF—B、PDGFR一仅、PDGFR—B的mR—NA转录水平都高于对照组（1．5609±0．1246比0．4057±0．0007，P〈0．05；0．4810±0．0733比0．26394±0．0070，P〈0．05；0．4963±0．0137比0．3038±0．0859，P〈0．05；1．1257±0．2490比0．0736±0．0185，P〈0．05）。结论血小板衍生生长因子（PDGF）-A、PDGF—B及其α、β受体活性表达的增强，参与了肺动脉高压肺血管重构的机制。
Objective To investigate the expression of PDGF-A,PDGF-B, PDGFR-α, PDGFR-β mRNA in lung of rat model of pulmonary hypertension induced by injection of monocrotaline and their role in pulmonary vascular remodeling. Methods Twenty-four SD rats, weighing 250-300 g, were randomly divided into 2 groups ： monocrotaline group （ MCT, n = 12）, control group （ Con, n=12）. The rats in MCT group received a single subcutaneous injection of monocrotaline （60 mg/kg）. After fed for 6 weeks, the expression of PDGF-A,PDGF-B, PDGFR-α, and PDGFR-β mRNA in lung tissue was detected by RT-PCR. Results In MCT group, the expression levels of PDGF-A, PDGF-B, PDGFR-αand PDGFR-β mRNA were higher than those in control group （ 1. 5609± 0. 1246 vs 0. 4057 ±0. 0007 ,P 〈 0.05 ;0.4810 ± 0.0733 vs 0.2639 ±0.0070,P 〈0.05;0.4963±0.0137 vs 0. 3038 ±0.0859,P 〈0.05;1. 1257 ±0. 2490 vs 0.0736 ± 0. 0185, P 〈 0.05 ）. Conclusion The higher expression level of PDGF-A, PDGF-B, PDGF R-α, and PDGFR-β may involve in the mechanism of pulmonary vascular remodeling in rat model of pulmonary hypertension induced by injection of monocrotaline.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第6期764-766,F0004,共4页
Chinese Journal of Experimental Surgery
基金
广东省科技计划资助项目（20088030301076）
关键词
肺动脉高压
肺血管重构
血小板衍生生长因子
Pulmonary artery hypertension
Pulmonary vascular remodeling
Platelet-derivedgrowth factor
