摘要
目的探讨温肾活血、益气升清的益筋方对实验性自身免疫性重症肌无力小鼠的治疗机制。方法采用重症肌无力患者的血清被动免疫小鼠，建立实验性自身免疫性重症肌无力模型。分组后分别给予益筋方、强的松灌胃21d，处死动物、采血。利用流式细胞技术，以T细胞及其亚群、IL-6为指标，观察益筋方、强的松对实验性重症肌无力小鼠细胞免疫和细胞因子的影响；检测胸腺Bcl-2的表达。结果益筋方、强的松均能升高CD3^＋，降低CD4^＋／CD8^＋比值；二者对IL-6无显著影响，均减低胸腺Bcl-2的表达。结论益筋方能通过影响T细胞及其亚群、Bcl-2，达到治疗重症肌无力的作用。
Objective The research studied the mechanism of Yijin Prescription with function of warming the kidney, promoting blood circulation, supplementing Qi and raising lucid Yang Qi of treating the mice myasthenia gravis （MG） caused by the disease of autoimmunity. Methods C57BL/6 mice were passively transferred by injected intraperitoneally with plasma of myasthenia gravis. Mice were administered by prednisone and Yijin Prescription, and killed after 21 days. By using the flowcytometric analysis, the effects of the Yijin Prescription and prednisone on the T lymphocyte and T lymphocyte subgroups and TL - 6 of the experimental myasthenia gravis of mice were observed respectively. The effect of Yijin Prescription and prednisone on the thymus was investigated by examining the expression of Bcl - 2 of thymus cells. Results The ratio of CD3^＋ was higher obviously than the control group and the model group; the ratio of CD4^＋ and CD8^＋ were lower significantly than the control group and the model group; the ratio CD4/CD8 was decreased significantly than the control group and the model group. The level of IL - 6 has no obvious difference with the control group, the expression of Bcl - 2 was lower than the control group and the model group. Conclusion Yijin Prescription can regulate T lymphocyte and T lymphocyte subgroup and Bcl -2 to treat MG.
出处
《中国医学创新》
CAS
2009年第16期14-16,共3页
Medical Innovation of China
基金
山东省中医药管理局2005-078课题.
