摘要
目的:观察不同穴位处方麦粒灸对失眠大鼠下丘脑5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)、去甲肾上腺素(NE)、多巴胺(DA)质量分数的变化,探讨麦粒灸治疗失眠症的作用机理。方法:将SD大鼠36只随机分为6组即对照组、模型组、安定组、麦粒灸1组(取百会、神道、心俞穴),麦粒灸2组(取三阴交、神门穴),麦粒灸3组(取申脉、照海穴),模型组以腹腔注射对氯苯丙氨酸(PCPA)建立大鼠失眠模型,安定组给予腹腔注射安定,麦粒灸各组分别按穴位给予麦粒灸治疗,每组连续治疗6 d后检测大鼠下丘脑5-HT、5-HIAA、NE、DA的变化。结果:模型组5-HT和5-HIAA质量分数明显低于对照组(P<0.01);麦粒灸各组5-HT、5-HIAA明显升高,NE、DA明显降低,与模型组和安定组比较差异有显著性(P<0.05,P<0.01);麦粒灸各组之间比较,麦粒灸2组优于麦粒灸1组(P<0.05),麦粒灸3组与麦粒灸1组、麦粒灸2组比较均无显著性差异(P>0.05)。结论:麦粒灸可能是通过提高失眠大鼠下丘脑5-HT、5-HIAA质量分数,降低NE、DA质量分数而改善睡眠;麦粒灸2组作用优于麦粒灸1组,说明取三阴交、神门穴治疗失眠症疗效更好。
Aim：To observe the effects of Moxibustion at different acupoints group on the contents of 5-hydroxytryptamine （5-HT）, 5-hydroxyindoleacetic acid （5-HIAA）, dopamine （DA）, and norepinephfine （NE） in the hypothalamus of insomnia rats to investigate the mechanism of Moxibustion treatment of insomnia. Methods： Thirty-six SD rats were randomly divided into six groups： controlled group, model group, diazepam group, grain-sized Moxibustion group Ⅰ （ Baihui （ DU20 ）, Shendao （ DU11）, Xinshu（ BL15 ） ）, grain-sized Moxibustion group Ⅱ （ Sanyinjiao （ SP6 ）, Shenmen （ HT7 ） ）, grain-sized Moxibustion group Ⅲ （ Shenmai （ BL62 ）, Zhaohai （KI6） ）. The model of insomnia rats were established by DL-P-Chlorophenylalanine（PCPA） peritoneal injection. The diazepam group rats were treated by diazepam peritoneal injection. Each grain-sized Moxibustion group rats were treated by Moxibustion at different acupoints. Monoamine neurotransmitters such as 5-HT, 5-HIAA, NE and DA in the hypothalamusof insomnia rats in each group were determine after 6 days continuously treatment. Results： After Moxibustion treatment the contents of 5-HT and 5-HIAA of the model group decreased significantly in contrast with controlled group, the contents of 5-HT and 5-H/AA increased obviously in the different grain-sized Moxibustion groups while the contents of NE and DA decreased significantly in contrast with model group and diazepam group（ P 〈0. 05 ,P 〈0. 01 ）. Among the grain-sized Moxibustion groups, group Ⅱ was better than the group Ⅰ（P 〈0. 05）, but there was no significant difference between the group Ⅲ and the other two groups （ P 〉 0.05 ）. Conclusion： The mechanism of grain-sized Moxibustion treatment of insomnia may be due to the increase of monoamine neurotransmitters such as 5-HT, 5-HIAA and the decrease of monoamine neurotransmitters such as NE, DA in the hypothalamus of insomnia rats. Grain-sized Moxibustion group Ⅱ was better than the group Ⅰ
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
北大核心
2009年第2期185-188,共4页
Journal of Jinan University(Natural Science & Medicine Edition)
基金
广东省中医药局科研基金资助项目(1050119)
关键词
失眠症
麦粒灸
穴位处方
单胺类神经递质
动物实验
insomnia
grain-sized Moxibustion
acupoint group
monoamine neurotransmitters
animal experiment
