摘要
目的燃煤污染型砷中毒是一种严重危害人体健康的地方病。本研究旨在观察贵州燃煤污染型砷中毒患者口腔黏膜脱落细胞及外周血相关基因表达的变化，探讨燃煤污染型砷中毒患者体内生物学标志，有助于地方性砷中毒的诊治与监控。方法2008年收集贵州省兴仁县交乐乡燃煤污染型砷中毒伴有皮肤表现的患者及非病区正常人外周全血及口腔黏膜脱落细胞，提取mRNA，以荧光实时定量PCR（realtime．PCR）方法比较研究砷中毒患者及健康正常人口腔黏膜脱落细胞及全血相关基因表达，同时采取外周静脉血检测肝功能变化。结果燃煤污染型砷中毒患者在仅有皮肤病变时肝功能检测已出现血清酶学改变，丙氨酸转氨酶明显升高[（36．9±17．4）／（25．8±2．4），t=0．019，P〈0．05]。与正常对照组比较，患者口腔黏膜脱落细胞中角蛋白10（K10）基因表达明显增加（3．6±0．9／1．8±0．7，t=0．041，P〈0．05），MT-1基因表达明显降低（0．8±0．5／3．9±0．4，t=0．028，P〈0．05）．c-myc、COX2、ER-α、AFP基因表达虽然也有所增加，但差异无统计学意义。结论口腔黏膜脱落细胞K10基因、MT-1基因表达改变可作为一个敏感的生物学标志物应用于燃煤污染型砷中毒的防治与监测，加强病区现症患者肝功能保护应成为燃煤型砷中毒防治工作中值得注意的重要环节。
Objective To observe the oxidative stress and skin disease related genes expression in cells exfoliated from buccal mucous and blood in patients with arseniasis caused by coal-burning pollution in Guizhou. Methods Buccal and blood cell samples were collected from arsenicosis patients and healthy subjects residing in Guizhou. Total RNA was isolated for related genetic transcript analysis. Real-time PCR （RT-PCR） was employed to detect the change of related gene expression. Serum indicators of liver injury, such as serum alanine aminotransferase （ALT）, were detected. Results Serum levels of ALT in arsenic patients were significantly higher than those in controls （36. 9 ± 17. 4 / 25. 8 ± 2. 4, t =0. O19,P 〈0. 05） even when no symptom was found in these patients. Buceal K10 gene was overexpressed （3.6 ±0. 9/1.8 ± 0. 7, t = 0. 041, P 〈 0. 05 ） and MT-1 expression was significantly lower （ 0. 8 ± 0. 5 / 3.9 ± 0. 4, t = 0. 028, P 〈 0. 05 ） in arsenic patients than that in control while the expressions of other genes were the same as those of controls. Conclusion The expressions of MT-1 and KIO gene in buceal mucous cells may be regarded as sensitive molecular markers for skin and hepatic pathologic changes in arsenic patients. Protection of liver function should be considered as an important step in arseniasis prevention and control.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2009年第21期1446-1449,共4页
National Medical Journal of China
基金
国家科技部国际合作重点项目（2005fd30640）
贵州省科技厅国际合作重点项目基金（2007400122）
关键词
砷
口腔黏膜
金属硫蛋白
基因表达
Arsenic
Mouth mucosa
Metallothionein
Gene expression
