摘要
研究了槲蕨(Drynaria roosii)孢子的无菌培养和常规繁殖方式,观察记录消毒方式、无机盐浓度和光照强度对槲蕨孢子萌发和配子体发育的影响。结果表明:槲蕨孢子接种后6～7d即能萌发,60d左右发育形成心形原叶体,100d左右开始形成孢子体幼苗;采用NaClO消毒5.5min左右是理想的消毒方式;在Knops培养基中孢子的萌发率最高,可达49%左右。低无机盐浓度的MS培养基有利于孢子萌发,但不利于配子体发育;黑暗条件下孢子能萌发但不能形成片状体。采用5%NaClO表面灭菌5.5min,1/2MS培养基在40μmol.m-2.s-1光照条件下孢子萌发和配子体发育良好。
The spore in vitro culture and routine propagation of Drynaria roosii were studied respectively. Tests were conducted to evaluate the effects of various sterilization procedures, medium and light intensity on spores of Drynaria roosii. The result exhibits that the spores germinated within 6 -7 days after inoculated in the medium, and sporophytes began to form in 100 days after inoculated. The maximum germination ratio was 49% obtained in Knop＇s medium. The low salt concentration of MS basal medium was helpful to spore germination, but inhibited the development of gametophytes. Spores can germinate in dark but can＇t develop to prothallial plate. Spores surface-sterilized for 5.5 min with 0.5% NaClO and inoculated in 1/2MS medium, were appropriate for Drynaria roosii cultivation with 40 μmol · m^-2 · s^-1 light intensity.
出处
《园艺学报》
CAS
CSCD
北大核心
2009年第5期711-716,共6页
Acta Horticulturae Sinica
基金
国家科技部项目(2005DKA21000,2006GH559901,2007AA021405)
北京市自然科学基金项目(7072045)
关键词
槲蕨
孢子萌发
无菌培养
Drynaria roosii Nakaike
spore germination
in vitro culture
