摘要
以‘富士’和‘金冠’苹果果实为试材,研究了β-半乳糖苷酶(β-Gal)和脂氧合酶(LOX)在果实发育、成熟和软化过程中的变化规律及采后乙烯调控对其活性的影响。结果表明:在果实发育后熟过程中,金冠果实β-Gal活性显著高于富士,在果实后熟软化期间这种差异尤为突出;而富士和金冠果实LOX活性呈相似的变化规律,虽然花后富士的活性高峰显著高于金冠,但之后其活性下降迅速,并一直低于金冠。采后苹果果实β-Gal和LOX均受乙烯调控,乙烯抑制剂1-MCP极显著地抑制β-Gal和LOX的活性,而乙烯利对二者活性起促进作用,但因品种耐藏性不同其促进效应不同。综合来看,苹果果实β-Gal和LOX活性表现相似的变化规律,并且β-Gal在果实贮藏初期受乙烯的调控作用较LOX显著,因此认为在苹果果实软化早期β-Gal的作用可能大于LOX。
For understanding the role of β-galactosidase （β-Gal） and lipoxygenase （LOX） during fruit development, ripening and softening, the fruits of apple （ Malus domestica Borkh. ） cultivars, ＇ Fuji＇ and ＇Golden Delicious＇ , were used to investigate the activity changes of β-Gal and LOX over this period, and during postharvest regulation by ethylene. The results indicated that the β-Gal activity in ＇ Golden Delicious＇ was markedly higher than that in ＇ Fuji＇ during fruit development and ripening and the difference was in particular significant during ripening and softening. The LOX activity in ＇Fuji＇ was dramatically increased and higher than that in ＇ Golden Delicious＇ after flowering, but its activity was markedly reduced and maintained lower than that of ＇ Golden Delicious＇ before fruit ripening. The β-Gal and LOX activities of apple fruit were markedly inhibited by 1-methycyclopene （1-MCP） and increased by ethephon. The changes of β-Gal and LOX activity showed a similar trend and the β-Gal be much easier to be regulated by ethylene than LOX. These results suggested that the change of β-Gal and LOX activities be correlated with the storage characteristic of apple fruit and β-Gal might have an important role in the early stage of fruit softening.
出处
《园艺学报》
CAS
CSCD
北大核心
2009年第5期631-638,共8页
Acta Horticulturae Sinica
基金
国家科技支撑计划项目(2007BAD79B05)
西北农林科技大学拔尖人才支持计划项目
