摘要
以毒物对青海弧菌Q67(Vibrio-qinghaiensissp.-Q67)发光抑制为毒性指标,基于短期微板毒性分析法(S-MTA法),建立了长期微板毒性分析法(L-MTA法),测定了吡虫啉(IMI)、抗蚜威(PIR)、啶虫脒(ACE)及敌百虫(DIP)等4种杀虫剂与氯霉素(CHL)、盐酸四环素(TET)、硫酸链霉素(STR)及硫酸巴龙霉素(PAR)等4种抗生素的短期(15 min)毒性和长期(12 h)毒性.采用短期毒性与长期毒性EC50之比(RSL)为指标,表征同一物质的毒性差异.结果表明:3种杀虫剂(IMI,ACE及PIR)的短期毒性与长期毒性差异不大(其RSL分别为0.84,0.81和1.11);杀虫剂DIP和2个抗生素(CHL及TET)的短期毒性与长期毒性差异明显(其RSL分别为11.76,9.67及154.38);另2个抗生素(STR和PAR)只有较强的长期毒性.
Taking bioluminescence inhibition of toxicants to Vibrio-qinghaiensis sp.-Q67 (Q67) as a toxicity indicator, a long-term microplate toxicity analysis (L-MTA) was developed based on short-term microplate toxicity analysis (S-MTA). The short-term (15 min) and lang-term (12 h) toxicities of four insecticides - imidacloprid (IMI), pirimicarb (PIR), acetamiprid (ACE), and dipterex (DIP) - and four antibiotics-chloramphenicnl (CHL), tetracyline hydrochloride (TET), streptomycin sulfate (STR), and paromomycin sulfate (PAR)-were determined by using the S-MTA and L-MTA, respectively. When the ratio of EC50 values from S-MTA and L-MTA (RSL) is used to depict the toxicity difference, three insecticides (IMI, ACE and PIR) show no differences between the toxicities from S-MTA and L-MTA (RSL are 0.84, 0.81 and 1.11, respectively), and the other insecticide (DIP) and two antibiotics (CHL and TET) present distinct differences (RSL are 11.76, 9.67 and 154.38, respectively). The other two antibiotics (STR and PAR) only have the long-term toxicities.
出处
《环境科学研究》
EI
CAS
CSCD
北大核心
2009年第5期589-594,共6页
Research of Environmental Sciences
基金
国家高技术研究发展计划(863)项目(2007AA06Z417)
国家自然科学基金项目(20577023,20777056)
全国优秀博士学位论文作者基金项目(200355)
关键词
微板毒性分析法
短期毒性
长期毒性
杀虫剂
抗生素
microplate toxicity analysis
short-term toxicity
long-term toxicity
insecticide
antibiotic