摘要
背景:同种异体骨材料经过灭菌和降低免疫原性等物理方式和化学方法处理后,要求其保留一定的生物活性和生物力学特性。目的:观察5种不同制备工艺对同种异体骨生物力学特性的影响。设计、时间及地点:对比观察实验,于2008-10在上海理工大学生物力学与康复工程研究所完成。材料:新鲜尸体的股骨,由上海亚朋生物技术有限公司合作医院提供。方法:将新鲜尸体的股骨经过预处理后,制成形状大小相同的骨块移植材料,采用γ射线辐照、高压冲洗后短超声、切割后长时间超声、化学处理后短超声和常规工艺5种不同制备工艺进行处理,经深低温冷冻保存1个月后取出,在Instron实验机上进行轴向压缩实验,加载过程为匀速准静态,速度为0.1mm/min。主要观察指标:同种异体骨轴向压缩下载荷-位移曲线和应力-应变曲线。结果:不同制备工艺下同种异体骨所承受的最大压缩载荷范围在1.1～2.1kN,其中,γ射线辐照骨承受的载荷最大,常规工艺处理骨承受的载荷最小,高压冲洗后短超声骨的承载能力与γ射线辐照骨的承载能力较接近。在骨的弹性限度范围内,化学处理后短超声骨的弹性变形区最小,刚度最大,切割后长时间超声骨的刚度最小,其余处理方法位于这两者之间。此外形状和结构相似的同种异体骨,应力-应变曲线的变化趋势相同,所承受的最大屈服应力值为4.5～9.5MPa。结论:不同制备工艺对骨的屈服应力、弹性应变量及弹性模量等生物力学参数均有不同程度的影响。
BACKGROUND： The organism active and biomechanical properties of bone are required to conserve after immune elimination and sterilization disposal using the physical and chemical methods. OBJECTIVE： To observe the biomechanical properties of allograft bone under the five class different disposal methods.
DESIGN,TIME AND SETING： A contrast experiment was performed at the Institute of Biomechanics and Rehabilitative Engineering, Shanghai University of Science and Technology in October 2008.
MATERIALS ： The thighbone of the fresh body was provided by Shanghai Yapeng Biotechnology Co., Ltd.
METHODS： The thighbone of the fresh body was cut into bone blocks with the same shape and dimension, disposed by using gamma-ray radiation, short time ultrasonic wave after high pressure irrigation, long time ultrasonic wave after incision, short time ultrasonic wave after chemistry method and conventional method and preserved at the deep-low temperature freezing. One month later, the compression test of bone block was carried out adopting 0.1 mm/min uniformity static state lode-velocity at the Instron machine.
MAIN OUTCOME MEASURES： The load-displacement curve and stress-strain curve of allograft bone in the axes-direction compression experiment.
RESULTS： The compression load of allograft bone under the five different disposal methods ranged from 1.1 KN to 2.1 KN; especially, the load was the strongest by using gamma-ray radiation, but the weakest by using conventional method; while, the load was similar between short time ultrasonic wave after high pressure irrigation and gamma-ray radiation. Bone elasticity was the lowest but rigidity was the highest by using short time ultrasonic wave after chemistry method; rigidity was the lowest by using long time ultrasonic wave after incision; other values were in the middle range. Allograft bone with the same shape and structure had the same changes of stress-strain curve, and maximal yield stress values located between 4.5 MPa and 9.5 MPa.
CONCLUSION： Differ
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第21期4098-4100,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
上海高校选拔培养优秀青年教师科研专项基金(571215)
博士科研启动费基金(10D214)~~
