摘要
目的探讨神经生长因子(NGF)对脊髓损伤保护作用的机制。方法采用Allen's法以25gcm致伤大鼠T_8脊髓,经蛛网膜下腔导管分别于术后即刻、1、2、3、4、8及24小时各注入NGF溶液,并以生理盐水组和正常组作对照。采用高效液相色谱法检测神经组织中兴奋性氨基酸即谷氨酸(Glu)、天冬氨酸(ASP)含量。结果与正常组相比较,Glu、ASP在伤后10分钟及8小时均显著升高,NGF治疗组伤后10分钟及8小时升高峰受到明显抑制。结论 NGF通过抑制脊髓损伤后兴奋性氨基酸的升高效应,抑制了兴奋性氨基酸的毒性作用,从而保护神经组织。
Objective To investigate the protective mechanisms of nerve growth factor (NGF) on spinal cord injury. Methods SCI of Wistar rats was performed by a 10g×2.5 cm impact on the posterior T_8 spinal cord, and a thin plastic tube was situated in subarachnoid space below the injury level for perfusion. The experimental animals received 60μg (20μl liquid) NGF purified from bovine seminal plasma at once, 1, 2, 3, 4, 8, 12, and 24 h after injury. An equal volume of normal saline was given to the rats of control group at the same time. In the experimental group, the injured spinal cord tissue was taken following treatment. The contents of the excitatory amino acids (Glu, Asp) were determined by high pressure liquid chromatography. Results The excitatory amino acid contents in the injured spinal cord were significantly increased at 10 min and 8 h after the injury as compared with those in control group. However, The peak values of the excitatory amino acid contents in NGF group were obviously lowered. Conclusion NGF can protect spinal cord against injury in vivo. One of mechanisms is that NGF prohibits neurotoxicity of the excitatory amino acids.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1998年第4期368-369,共2页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金(No.39470701)
关键词
脊髓损伤
兴奋性
氨基酸
神经生长因子
Spinal cord injury
Nerve growth factor
Excitatory amino acid
