摘要
目的：为明确卵巢早衰者外周血Ｔ淋巴细胞亚群及补体的特点。方法：运用免疫酶组化技术———碱性磷酸酶—抗碱性磷酸酶（ＡＰＡＡＰ）法，检测卵巢早衰（ＰＯＦ）患者（ｎ＝３０）、自然绝经后妇女（ｎ＝２０）及健康育龄妇女（ｎ＝３０）的外周血Ｔ淋巴细胞亚群，同时检测其补体水平。结果：①健康育龄妇女、卵巢早衰者及自然绝经者在外周血成熟Ｔ淋巴细胞总体水平是一致的。②ＰＯＦ患者及自然绝经后妇女的ＣＤ＋８、ＣＤ＋１６Ｔ淋巴细胞数明显升高（Ｐ＜０．０５），ＣＤ＋４／ＣＤ＋８明显降低（Ｐ＜０．０５），卵巢早衰者出现的闭经、衰老与体内存在的免疫异常，不仅与细胞的相对数量有关，还与其调节功能有关，即淋巴细胞亚群失衡有关。③自然绝经者Ｃ３、Ｃ４明显高于健康育龄妇女（Ｐ＜０．０５），卵巢早衰者Ｃ４显著高于健康育龄妇女（Ｐ＜０．０５）。结论：卵巢早衰病因之一可能是自身免疫性卵巢炎。卵巢早衰者与自然绝经者体内细胞免疫、补体水平相似，卵巢早衰者的免疫调节、免疫应答均处于衰老状态。
Objective:Our purpose was to understand the characteristics of peripheral T lymphocytes subpopulations and complements of POF women.Methods:Peripheral T lymphocytes subpopulations(CD+3CD+4CD+8CD+16)of POF(n=30),postmenopaused women(n=20)and health women(n=31)were tested by APAAP method. Complements(C3,C4,CH50)were measured at the same time. Results:① The total level of peripheral matured T lymphocytes showed no difference in three groups. ②CD+8percentage and CD+16percentage of POF and postmenopaused cases were higher than that of healthy cases (P<0.05). Rates of CD+4/CD+8 of former were much lower than that of latter(P<0.05). Phenomena of amenorrhoea, aging, and abnormal of immunity of POF were associated with the amount of lymphocytes and the regular function. ③ Not only C3 level and C4 level of postmanopaused cases but also C4 level of POF were much higher than that of healthy women (P<0.05). Conclusions: One of the cause of POF might be autoimmunal ophorotitis. The celular immunity and complement level of POF were similar to that of postmenopaused women. The immunoregulation and immunoresponse of POF were in aging situation.
出处
《中山医科大学学报》
CSCD
1998年第1期53-57,共5页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
孙逸仙纪念医院科研基金
关键词
卵巢功能早衰
免疫学
T淋巴细胞
补体
ovarian failure, premature/immunology
Tlymphocyte
complement
