摘要
目的:观察电刺激丘脑底核(STN)及微电泳γ-氨基丁酸(GABA)、谷氨酸(Glu)及其对应的拮抗剂等对大鼠黑质网状部(SNr)神经元放电的影响,进一步探讨电刺激治疗帕金森病(PD)的机制。方法:应用细胞外记录的方法观察电刺激STN和微电泳药物对SNr神经元放电的影响。结果:低频电刺激时,SNr的放电频率无显著变化(P>0.05),随着刺激频率的增加,大多数SNr神经元的放电活动受抑制,受抑制神经元放电频率较刺激前差异显著(P<0.05)。Glu对SNr有紧张性兴奋作用,GABA对SNr有紧张性抑制作用。被高频电刺激(HFS)STN抑制的SNr神经元中,有80%在微电泳BIC的基础上进行STN-HFS不再出现抑制反应。结论:采用电刺激治疗PD以STN为靶核团时,应选用HFS。STN-HFS可能主要通过GABA的抑制作用来调节SNr的异常活动。
To research the spontaneous firing activities during different-frequency stimulation of subthalamic nucleus and microelectrophoresis GABA, Glu and their antagons respectively, approaching the mechanism of DBS in the treatment of Parkiuson＇ s disease further. Methods： Using extracellular recording to investigate the effect of different-frequency stimulation of STN and microelectrophoresis several drugs on the spontaneous firing activities of the SNr neurons. Results： For STN stimulation at low frequency, there was no difference on the spontaneous firing acfivefies of SNr neurons between pro-stimulation and meta-stimulation（ P 〉 0.05）. With the increasing of stimulation frequency, most of the SNr neurons were inhibited. While during the STN stimulation frequency at high-frequency, the firing rates of inhibited SNr neurons were changed（ P 〈 0.05）. Glu had catatonic excitement effect on the SNr neurons, whereas GABA had tonic inhibition effect. 80% of SNr neurons which were inhibited by STN-HFS were not inhibited by STN-HFS on the basis of excitatory effect of BIC. Conclusion： To treat the motor symptoms of PD, when STN is selected as the target nucleus, the electrical stimulation with high-frequency should be chosen. It is possible that STN-HFS modulate the activity of SNr by inhibitory effect of GABA predominantly.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2009年第2期233-236,共4页
Chinese Journal of Applied Physiology
关键词
微电泳
帕金森病
丘脑底核
microelectrophoresis
Parkinson＇ s disease（PD）
STN
