摘要
建立了离子交联法制备恩诺沙星壳聚糖纳米粒的方法,并优化了工艺条件,以低温超速离心法评价了恩诺沙星壳聚糖纳米粒包封率。利用透射电子显微镜分析了恩诺沙星壳聚糖纳米粒的显微形态,以透析法测定其体外释放度。结果表明:恩诺沙星壳聚糖纳米粒呈球形,外观形态饱满,表面光滑,分散性良好,平均粒径为(131.1±10.2)nm,平均包封率51.2%±2.9%。相比恩诺沙星原料药,恩诺沙星壳聚糖纳米粒在1 h内减少约60%药物的释放,24 h总释放度为79.9%。说明利用离子交联法制备恩诺沙星壳聚糖纳米粒快速、简便、操作性强,恩诺沙星壳聚糖纳米粒制剂缓释性能强,适于作为新剂型在水产药物中应用。
Enrofloxacin chitosan nanoparticles(ENR-CS-NPs) were prepared by using ionic gelation method. The preparing method was optimized through orthogonal design. Encapsulation efficiency was determined by the way of ultracentrifuge at 4℃ and the content of enrofloxacin was determined by UV. The microstructure of nanoparticles was observed with transmission electron microscopy. In vitro release degree was measured by dialysis method. The nanoparticles observed by TEM were spherical with a mean particle size of ( 131. 1 ± 10.2) nm. The encapsulation efficiency of ENR-CS-NPs was 51.2% ± 2.9%. The drug release degree of ENR-CS-NPs of about 60% decreased in 1 hour compared with ENR solution and the total drug release degree in 24 h is 79.9% with the sustained releasing behavior. Results showed that ionic gelation method is applicable to the preparation of ENR-CS-NPs.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2009年第3期321-326,共6页
Journal of Shanghai Ocean University
基金
国家"十一五"科技支撑计划(2006BAD03B04)
农业公益性行业科研专项(200803013)
关键词
恩诺沙星
壳聚糖
纳米粒
体外释放
enrofloxaein
chitosan
nanoparticles
in vitro release