期刊文献+

恩诺沙星壳聚糖纳米粒的制备及其体外释药特性研究 被引量:8

Preparation and release characteristics of enrofloxacin chitosan nanoparticles in vitro
下载PDF
导出
摘要 建立了离子交联法制备恩诺沙星壳聚糖纳米粒的方法,并优化了工艺条件,以低温超速离心法评价了恩诺沙星壳聚糖纳米粒包封率。利用透射电子显微镜分析了恩诺沙星壳聚糖纳米粒的显微形态,以透析法测定其体外释放度。结果表明:恩诺沙星壳聚糖纳米粒呈球形,外观形态饱满,表面光滑,分散性良好,平均粒径为(131.1±10.2)nm,平均包封率51.2%±2.9%。相比恩诺沙星原料药,恩诺沙星壳聚糖纳米粒在1 h内减少约60%药物的释放,24 h总释放度为79.9%。说明利用离子交联法制备恩诺沙星壳聚糖纳米粒快速、简便、操作性强,恩诺沙星壳聚糖纳米粒制剂缓释性能强,适于作为新剂型在水产药物中应用。 Enrofloxacin chitosan nanoparticles(ENR-CS-NPs) were prepared by using ionic gelation method. The preparing method was optimized through orthogonal design. Encapsulation efficiency was determined by the way of ultracentrifuge at 4℃ and the content of enrofloxacin was determined by UV. The microstructure of nanoparticles was observed with transmission electron microscopy. In vitro release degree was measured by dialysis method. The nanoparticles observed by TEM were spherical with a mean particle size of ( 131. 1 ± 10.2) nm. The encapsulation efficiency of ENR-CS-NPs was 51.2% ± 2.9%. The drug release degree of ENR-CS-NPs of about 60% decreased in 1 hour compared with ENR solution and the total drug release degree in 24 h is 79.9% with the sustained releasing behavior. Results showed that ionic gelation method is applicable to the preparation of ENR-CS-NPs.
出处 《上海海洋大学学报》 CAS CSCD 北大核心 2009年第3期321-326,共6页 Journal of Shanghai Ocean University
基金 国家"十一五"科技支撑计划(2006BAD03B04) 农业公益性行业科研专项(200803013)
关键词 恩诺沙星 壳聚糖 纳米粒 体外释放 enrofloxaein chitosan nanoparticles in vitro release
  • 引文网络
  • 相关文献

参考文献22

  • 1朱模忠主编..兽药手册[M].北京:化学工业出版社,2002:520.
  • 2邓永强,汪开毓,黄小丽.水产药物制剂生产存在的问题与对策[J].科学养鱼,2004,26(7):42-42. 被引量:4
  • 3杨先乐主编,农业部《新编渔药手册》编撰委员会编..新编渔药手册[M].北京:中国农业出版社,2005:646.
  • 4中国兽药典委员会编..中华人民共和国兽药典 一部[M].北京:化学工业出版社,2000:390.
  • 5林爱华,平其能.壳聚糖载药纳米粒研究进展[J].中国药业,2006,15(21):25-27. 被引量:12
  • 6Behrens I, Pena A I, Alonso M J, et al. Comparative uptake studies of bioadhensive and non-bioadhensive nanoparticles in human intestinal cell lines and rats: the effect of mucus on particle adsorption and transport[ J ]. Pharmaceutical Research ,2002,19 (8) :1185. 被引量:1
  • 7袁晓佳,张志荣,何勤.胸腺五肽三甲基壳聚糖纳米粒包封率的测定[J].华西药学杂志,2005,20(2):131-133. 被引量:9
  • 8方开泰,马长兴著..正交与均匀试验设计[M].北京:科学出版社,2001:248.
  • 9黄宣运,胡鲲,方伟,金怡,杨先乐.环丙沙星偶联物的制备及其产物的鉴定[J].上海水产大学学报,2008,17(5):585-590. 被引量:7
  • 10Shilpa Sant,Veronique Nadeau, Patrice Hildgen. Effect of porosity on the release kinetics of propafenone-loaded PEG-g-PLA nanoparticles [ J ]. Journal of Controlled Release,2005,107 : 203 - 214. 被引量:1

二级参考文献164

共引文献104

同被引文献98

引证文献8

二级引证文献29

;
使用帮助 返回顶部