摘要
采用ISSR分子标记技术对吉林省9个骨干玉米自交系进行了聚类分析。结果表明:全部供试自交系均可获得清晰、可辨且多态性丰富的谱带;根据聚类树分析,在相似系数0.28处,可将它们分为4个类别:Ⅰ类Mo17、吉842、龙抗11、1037;Ⅱ类铁7922和M67;Ⅲ类吉818和吉853;Ⅳ类444。这种划分基本与供试自交系的血缘系统相一致,因此ISSR是一项适于对玉米自交系杂种优势群进行划分的有效分子标记技术。
Nine key maize inbred lines from Jilin province were clustered based on the ISSR molecular marker technique. Results indicated that: clear, detectable and abundant polymorphism bands were ob- tained in these tested inbred lines; According to the cluster tree, 4 groups, namely Ⅰ cluster: Mo17, Ji842 and Longkang11 ; Ⅱcluster: Tie7922, M67; Ⅲ cluster: Ji818 and Ji853; Ⅳ cluster: 444 were obtained from them which were primarily identical with their origin system. Therefore ISSR is an available molecular marker technique for grouping maize inbred lines.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2009年第2期131-134,共4页
Journal of Jilin Agricultural University
基金
国家自然科学基金项目(30225003)
吉林农业大学博士科研启动基金(2007004)