摘要
降低细胞内的氧气含量是提高莱茵衣藻产氢效率的重要手段之一。研究首次尝试将豆血红蛋白基因lba转入衣藻叶绿体中表达,利用豆血红蛋白具有与氧可逆结合的特性,期望降低转基因衣藻细胞内的氧气含量,达到提高衣藻产氢效率的目的。实验结果证明,lba成功转入到衣藻叶绿体中,且对其生长没有产生显著影响,为下一步调控lba在衣藻叶绿体中表达活性和提高衣藻产氢效率奠定了实验基础。
To decrease the oxygen content in the cell is a key method to improve hydrogen production in Chlamydomonas reinhardtii. A new approach was developed by transforming the leghemoglobin gene lba, which has high affinity to oxygen, into the chloroplast of C. reinhardtii to get a low dissolved oxygen in the cell and result into improvement of H2 ase activity and n2 yield. The results showed that lba was successfully transformed into the chloroplast of C. reinahrdtii strain 849 and did not affect its growth significantly. The work paved the road for further regulation of lba expression in the chloroplast to improve of hydrogen production of C. reinahrdtii.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2009年第5期66-71,共6页
China Biotechnology
基金
上海市浦江人才计划(06PJ14075)
上海市教育委员会科研创新项目(08YE66)
上海市教育委员会重点学科建设项目(J50401)
上海市科委基础性研究重点项目(03JC14057)资助项目
关键词
莱茵衣藻
豆血红蛋白
生物制氢
叶绿体转化
Chlamydomonas reinhardtii Leghemoglobin Bio-hydrogen production Chloroplast transformation
