摘要
目的:研究不同浓度重铬酸钾(K2Cr2O7)对人肺腺癌A549细胞的增殖抑制效应以及对O6-甲基鸟嘌呤-DNA甲基转移酶(hMGMT)蛋白表达的影响。方法:用四甲基偶氮唑蓝(MTT)比色法测定9种不同浓度K2Cr2O7(0.000、0.010、0.040、0.160、0.312、0.625、1.250、2.500、5.000μmol/L)对A549细胞的增殖抑制率;用损伤修复试验观察在不同浓度K2Cr2O7作用下人肺腺癌A549细胞的划痕修复情况;以β-actin为参照,应用Western blotting技术检测不同浓度K2Cr2O7对人肺腺癌A549细胞hMGMT表达的影响。结果:MTT比色法检测显示,大于0.625μmol/L浓度组K2Cr2O7可抑制细胞活性,与空白对照组比较,差异有统计学意义(P<0.05)。损伤修复试验显示,与空白对照组比较,0.625、1.250、2.500和5.000μmol/L的K2Cr2O7抑制细胞损伤修复,而0.312、0.160、0.040μmol/L的K2Cr2O7促进细胞损伤修复。Western blotting显示,0.312、0.160、0.040μmol/L的K2Cr2O7对hMGMT蛋白的表达具有促进作用,0.625、1.250、2.500和5.000μmol/L的K2Cr2O7对hMGMT蛋白的表达具有抑制作用。结论:低浓度的K2Cr2O7对人肺腺癌A549细胞的增殖、损伤修复和hMGMT蛋白的表达呈现低剂量兴奋效应;高浓度K2Cr2O7对hMGMT蛋白的表达则呈现抑制作用。
Aim：To investigate the effects of potassium dichromat on human lung adenoearcinoma A549 cell line in proliferation and hMGMT protein expression. Methods：Nine different concentrations of Potassium dichromat were administrated with methyl thiazolyl tetrazolium （MTT） assay to measure the inhibiting rates of cell growth. Wound-healing assay was applied to observe the condition of wound-healing. Western blotting was applied to determine the expression level of hMGMT protein. ReSults： The MTT assay showd that 0. 625 μmol/L, 1. 250 μmol/L, 2. 500 μmol/L and 5. 000 μmol/L K2Cr207 could restrain cell growth. Compared with the control group,the difference of cellular multiplication was different （P 〈 0.05）. Wound-healing assay showed K2Cr207 can suppress cell repair with concentration of 0. 625μmol/L, 1. 250 μmol/L, 2. 500 μmol/L and 5. 000 μmol/L, and promote cell repair with concentration of 0. 312 Ixmol/L, 0. 160 μmol/L and 0. 040 μmol/L. Western blotting showed K2 Cr207 can promote the expression of hMGMT protein with concentration of 0. 312 μmol/L, 0. 160 μmol/L and 0. 040 μmol/L, and suppress the expression of hMGMT protein with concentration of 0. 625 μmol/L, 1. 250 μmol/L , 2. 500 μmol/L and 5. 000 μmol/L. Gonlusion： Low concentration of K2Cr207 showed hormisis phenomena of cellular multiplication,Wound-healing and hMGMT protein expression in human lung adenocarcinoma A549. Meanwhile, high concentration of K2 Cr207 could inhibit cellular multiplication, Wound-healing and hMGMT protein expression in human lung adenocarcinoma A549.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2009年第3期572-575,共4页
Journal of Zhengzhou University(Medical Sciences)
