摘要
目的观察睡眠剥夺对小鼠空间学习与记忆以及海马突触素Ⅰ(synapsinⅠ)表达的影响。方法20只健康雌性C57BL/6J小鼠随机分为两组:(1)剥夺睡眠组(SD,n=10),采用"轻触法"剥夺小鼠睡眠;(2)普通鼠笼对照组(CC,n=10)。采用Morris水迷宫进行航行定位实验测定各组小鼠的平均逃避潜伏期、游泳速度,并进行空间探索实验测定动物在月台所在象限时间的百分比。通过Western blot方法检测小鼠海马结构突触素Ⅰ的表达。结果睡眠剥夺组鼠到达月台的平均潜伏期大于对照组,而在月台所在象限时间的百分比明显低于对照组(P<0.05)。睡眠剥夺组鼠海马结构突触囊素Ⅰ的相对含量较对照组低(P<0.01)。结论睡眠剥夺可能通过影响海马的突触可塑性而降低实验动物的空间学习和记忆能力。
Objective To examine the effects of sleep deprivation （SD） on learning and memory and hippocampal synapsin Ⅰ level in mice. Methods Twenty female C57BL/6J mice were randomly divided into 2 groups：sleep deprivation group（SD,n=10） and normal cage control group （CC,n= 9）. All the animals were subjected for Morris Water Maze to test mean escape latency, swimming speed and percentage of time spent in the target quardrant. Synapsin Ⅰ level in hippocampus was tested with western blot. Results Compared with control group, sleep deprivation group had longer mean escape latency to reach the platform（P〈 0.05）and spent less time in the target quadrant （P〈0.05）. Results of western blot for synapsin I revealed that the synapsin Ⅰ level in the hippocampal formation was significantly lower in sleep deprivation group（P〈0.01）. Conclusion Sleep deprivation impairs spatial learning and memory ability with impeding the synaptic increasing in hippoeampal formation.
出处
《重庆医学》
CAS
CSCD
北大核心
2009年第10期1153-1154,共2页
Chongqing medicine
基金
重庆市科委自然科学基金资助项目(CSTC
2008BB5014)
第三军医大学科研基金资助项目(2007XG063)
关键词
睡眠剥夺
学习
记忆
突触素Ⅰ
sleep deprivation
learning
memory
synapsin Ⅰ
