摘要
采用高效液相色谱法对爬山虎红色素中原花青素的含量进行了测定。色谱柱：C18柱（日本岛津5μm；150mm×4．6mm）,柱温：25℃，流动相：V（甲醇）：V（水）=90：10，检测波长：280nm，进样量：20止，流速：1ml／min。结果表明在0．50-7．5μg／mL范围内具有良好的线性关系，回收率为99．96％，相对标准偏差为1．0％，原花青素的平均含量为6．8μg／mL。结论是本方法简便、快速、准确、可靠，可为爬山虎叶子提取物红色素中原花青素的含量测定的方法。
The content of oanthocyanidins in red pigment of leafage of Parthenocissus tricuspidata Planch was determined by HPLC on a Shirnadzu C 18 column （5μm; 150mm×4.6mm） with the mobile phase of 90% methanol and 10% water. The column temperature, flow rate and detection wavelength were 25℃, 1 ml/min and 280 nm, respectively. The results showed that there was a good linear relationship between absorption and proanthocyanidins content within the range of 0.50-7.5μg/mL. Recovery rate and RSD of the method were of 99.96 % and 1.0%, respectively. The average content of proanthocyanidins was 6.8μg/mL. This method is simple, fast, accurate and reliable, suitable for determination of proanthocyanidins content in red pigment from leafage of Parthenocissus tricuspidata Planch.
出处
《现代食品科技》
EI
CAS
2009年第5期566-567,497,共3页
Modern Food Science and Technology
基金
山西医科大学大学生创新基金
关键词
原花青素
爬山虎
HPLC
Proanthocyanidins, Parthenocissus tricuspidata Planch, HPLC.
