摘要
目的观察哮喘大鼠生长相关癌基因α(GROα)蛋白的表达水平及布地奈德对其的影响,探讨GROα和中性粒细胞(PMN)的相关性。方法采用哮喘大鼠模型,将30只SD大鼠随机分成哮喘组、布地奈德治疗组、正常对照组,分离纯化血PMN,应用免疫组化法测定支气管壁和血PMN中GROα蛋白的表达水平,计数肺组织PMN数目。结果与对照组相比,哮喘组和治疗组的GROα蛋白的平均光密度值(OD值)在支气管壁(依次为0.138±0.009、0.114±0.007、0.077±0.010)和血PMN(依次为0.211±0.017,0.136±0.010,0.081±0.008)中差异均有统计学意义(P均<0.01);与哮喘组相比,治疗组的GROα蛋白的平均光密度值在支气管壁和血PMN中差异均有统计学意义(P均<0.01)。三组PMN计数(对照组6.70±2.41,哮喘组26.00±6.54,治疗组196.80±25.73)两两比较差异均有统计学意义(P均<0.05)。GROα蛋白的表达水平与肺组织PMN计数在哮喘组和对照组之间呈显著正相关(n=19,r=0.865,P<0.01)。结论哮喘时支气管壁和PMN的GROα蛋白的表达水平及肺组织PMN计数增加,它们参与了哮喘发病的炎症过程;布地奈德部分通过抑制GROα蛋白的表达从而减轻气道炎症,但能加剧PMN在肺组织中聚集;GROα蛋白可能与PMN在肺组织中聚集密切相关。
Objectives To observe the expressions of growth-related oncogene-α （GRO-α） protein in rat asthma model and how it is affected by budesonide. To investigate the relationship between GRO-α and polymorphonuclear leukocyte （PMN）. Methods Thirty （30） rat asthma model were randomly and equally divided into three groups, including asthma group （group A）, control group （group C） and budesonide treated group （group B）. Blood PMN were isolated and purified. The expression of GRO-α protein was detected by immunohistochemical method in bronchial wall and in blood PMN. Numbers of PMN in lung tissue was calculated. Results 1. Both levels of GRO-α protein in group A and group B were higher than that of group C in bronchial wall （0.138 ± 0.009, 0.114 ± 0.007, 0.077 ± 0.010, Optical density, respectively） and in blood PMN （0.211 ± 0.017, 0.136 ± 0.010, 0.081 ± 0.008, Optical density, respectively） （ all P 〈 0.01 ）. 2. There was significant difference both in levels of GRO-α protein in bronchial wall and in blood PMN between group A and group B （both P 〈 0.01 ）. 3. There were significant difference in number of PMN in lung tissue among three groups （6.70 -± 2.41 in group C, 26.00 ± 6.54 in group A, 196.80 ± 25.73 in group B, respectively; P 〈 0.05, P 〈 0.01, respectively）. 4. Positive correlation between GRO-α protein expression in bronchial wall and numbers of PMN in lung tissue was found in group A and group C （n = 19, r = 0.865, P 〈 0.01）. Conclusion Either the levels of GRO-α protein in bronchial wall and in blood PMN or the number of PMN in lung tissue was increased in asthma rat, which may involve in inflammation of asthma, Budesonide may be able to lessen inflammation of asthma via reducing production of GRO-α protein. Budesonide can also induce PMN influxing into lung tissue, which was correlated with GRO-α protein.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2009年第5期472-475,共4页
Journal of Clinical Pediatrics
基金
浙江省卫生厅基金资助项目(No.2007-B238)
温岭市科技局基金资助项目(No.2006-53)
关键词
哮喘
生长相关癌基因α
免疫组化
布地奈德
大鼠
asthma
growth-related oncogene-α
immunobistochemieal method
budesonide
rat
