摘要
随机选取中豆29×中豆32重组自交系群体中165个家系作为2年田间试验材料,分析大豆单株产量、产量构成因子及倒伏性的相关性和遗传效应,并检测各性状QTL。结果表明,38个与产量、产量构成因子及倒伏性状等有关的QTL主要集中在C2、F和I连锁群。表型相关分析结果与QTL定位结果一致。在F连锁群上,2年均检测到倒伏QTLqLD-15-1,解释的表型变异超过20%,与百粒重和分枝荚数QTL分别位于相同和相邻标记区间,表明产量相关性状与倒伏性存在一定的关联。在I连锁群上,每荚粒数QTL和二、三、四粒荚数QTL不仅于同一位置,解释的表型变异为32%-65%,并且2个年份均重复出现,每荚粒数和四粒荚数QTL与二、三粒荚数QTL的增效基因分别来自不同的亲本。这4个粒荚性状QTL的共位性与表型相关分析结果一致,证实每荚粒数和四粒荚数与二、三粒荚数分别由不同的机制调控。
To date, more than 80 QTLs for yield have been mapped on 18 linkage groups of soybean （Glycine max L. Merr.） using over 20 segregation populations. However, only QTLs that expressed stably in different populations and environments are of great value for soybean breeding. Except for QTLs associated with seed weight, there are few reports on QTLs for other yield-related traits. The objectives of this study were to detect yield-related QTLs in soybean and to compare linkage groups obtained from different mapping populations. A total of 165 recombinant inbred lines （RILs） derived from a cross between Zhongdou 29 and Zhongdou 32 were used to analyze the relationships of yield per plant, yield components, and lodging percentage as well as their genetic effects, and to map the QTLs in 2006-2007. Thirty-eight QTLs for yield, seed and pod traits, and lodging related traits were detected using composite interval mapping （CIM） method. Most of the QTLs were located on C2, F, and I linkage groups. QTL qLD-15-1 for lodging percentage on F linkage group was detected in both years, which explained more than 20% of the phenotypic variation. This QTL had the same or adjacent marker intervals to QTLs for 100-seed weight and pod number on branch, showing correlation between yield-related traits and lodging. On I linkage group, the QTLs for seeds per pod, two-seed pods, three-seed pods, and four-seed pods were detected on the same position across years and explained 32-65% of the phenotypic variation. The positive alleles at loci of seeds per pod, four-seed pods, two-seed -pods, and three-seed pods were from different parents. The four QTLs for seed and pod traits mapped on the same position, which was consistent with the correlation analysis result of phenotypic traits. This suggests that the regulatory mechanisms of the four traits for seed and pod are different.
出处
《作物学报》
CAS
CSCD
北大核心
2009年第5期821-830,共10页
Acta Agronomica Sinica
基金
国家自然科学基金项目(30871554和30671313)
国家高技术研究发展计划(863计划)项目(2006AA1000104)
国家“十一五”科技支撑计划项目(2006BAD01A04-3)
转基因生物新品种培育重大专项(2008ZX08004-005)资助
