摘要
目的:探讨猫爪草皂苷(Radix ranunculus ternate saponins,RRTS)对人结肠癌LoVo细胞的增殖和凋亡作用及作用机制。方法:按猫爪草皂苷浓度分为1、10、100、500μg.mL-14个处理组和对照组(未加猫爪草皂苷),处理LoVo细胞不同时间后,倒置显微镜观察细胞形态;采用MTT法检测细胞增殖的抑制情况;流式细胞仪(FCM)分析细胞周期和凋亡率。结果:与对照组比较,猫爪草皂苷处理组可使细胞明显变圆、体积缩小、脱壁细胞增多;MTT法检测显示,猫爪草皂苷处理组随着浓度的增加和作用时间延长,对LoVo细胞的增殖抑制作用增强,并呈时间、剂量依赖性(P<0.001);随着浓度的增加凋亡率增加,药物组与对照组比较差异有显著性(P<0.001);流式细胞仪细胞周期分析图可看出细胞阻滞在G1期,随着给药浓度的增加,细胞凋亡峰(AP峰)越来越明显(图1);激光共聚焦扫描技术观察显示,细胞经皂苷作用4h、8h、12h后,线粒体膜电位下降(P<0.05)。结论:猫爪草皂苷可导致人结肠癌LoVo细胞线粒体膜电位下降,具有明显抑制增殖和诱导凋亡作用。
Objective ：To explore the regulatory effect of radix ranunculus ternate saponins（RRTS） on proliferation and apoptosis in human colon carcinoma LoVo cells. Methods：The LoVo ceils were divided into 4 treatment groups （1, 10, 100,500μg · mL-1 RRTS） and blank control group. The morphological alterations of LoVo cells after treated for different time were observed by inverse microscopy, the inhibition of cell proliferation was detected by MTT assay, the cell cycle phase was analyzed and the apoptosis was inspected by flow cytometry; LCSM（ laser confocal scanning microscopy） was used to observe the affection of RRTS on the Δψm （ mitochondria membrane potential）. Results： After being treated with RRTS, LoVo cells grew round and small obviously and were against the wall under the inverse microscopel with the increasing of the concentration of RRTS and the elongation of the treated time, the LoVo cell growth was inhibited, the inhibition of proliferation of LoVo cells increased in a time-and dose-dependent manner（ P 〈 0.001 ） ; the apoptotic rates of the dosedependent treated groups increased significantly vs the control＇s（ P 〈 0. 001 ） ; cell cycle was blocked in G1 phase, and typical sub-diploid peaks were found by FCM analysis at different dosage groups; the mitochondria membrane potential decreased after treated by RRTS for 4, 8, 12h（ P 〈0.05）. Conclusion： RRTS could weaken the mitochondria membrane potential, significantly inhibit the growth and induce the apoptosis of LoVo cells.
出处
《中华中医药学刊》
CAS
2009年第5期1079-1081,共3页
Chinese Archives of Traditional Chinese Medicine
