摘要
本实验以 L-赖氨酸产生菌钝齿棒杆菌 (coryne bacterium crenatum) E2 2 6(HS+、ABCr、Metr、TAr、ESr)为出发菌株 ,经甘氨酸、青霉素 ,溶菌酶作用形成原生质体 ,并对原生体进行硫酸二乙酯(DES)的诱变处理 ,然后对大量的再生突变株进行初筛、复筛 ,获得了高产稳定菌株 D60～ 10 9,其发酵液中 L-赖氨酸含量较出发菌株提高了 19.4 %。并且又对发酵培养基通过均匀设计方法进行优化组合 ,选择了最佳配比 ,使 D60～ 10 9菌株发酵液中 L -赖氨酸含量由出发菌株的 68.2 mg/ ml提高到 91.7mg/ ml,提高了 34 .5% ,并且发酵液中不含其它杂酸 。
Coryne bacterium crenatum E 226 (HS +, AEC r , Met r, TA r , ES r) was used as initial strain. The protoplasts were prepared by glycine Penicillin and Lysozyme. The high yield stable strain D60～109 was obtained by DES irradiation of protoplasts and screening of the fermention of many regenerative mutants. Compared with E 226 , the yield of lysine in fermention broth was increased by 19.4%. The optimal medium of fermention was obtained by Uniform Design, the strain D 60～109 produced large amounts of L lysine(91.7mg/ml) more than parental strain E 226 (68.2mg/ml) (34.5% in percentage) and other amino acids in the fermention broth didn′t exist by the automatic amino acid analyzer, which benefited to further isolation.
出处
《药物生物技术》
CAS
CSCD
1998年第1期17-20,共4页
Pharmaceutical Biotechnology
关键词
诱变
发酵
赖氨酸
硫酸二乙酯
Coryne bacterium crenatum , Protoplast, Mutation, Uniform design
