摘要
目的:探讨结肠Cajal间质细胞(ICC)、干细胞因子(SCF)在溃疡性结肠炎(UC)发病中的作用。方法:将BALB/c小鼠随机分为正常对照组(n=8)和UC组(n=24),于造模后1、4、7天分别处死UC组8只小鼠,取远端结肠,用Westernblot方法检测SCF的表达;免疫组化检测结肠ICC的变化,并进行结肠组织学评分。结果:结肠组织学评分在7天达最高值(P<0.05)。与正常小鼠比较,UC小鼠结肠肌间ICC表达明显减少(P<0.05),结肠SCF的表达也显著减少(P<0.01),SCF与ICC呈正相关(UC4天时,r=0.862,P=0.027;UC7天时,r=0.948,P=0.004)。结论:UC小鼠远端结肠组织内SCF表达下降,伴随ICC数量减少,提示UC病变可能与SCF/Kit系统下调有一定的相关性。
Objective：To investigate the expression of interstitial cells of Cajal （ICC）and stem cell factor （SCF）in mice colon with ulcerative colitis （UC）. Methods：Thirty-two male health adult BALB/c mice were divided randomly into control group and UC groups. Eight mice of the UC groups were sacrificed after 1,4 and 7 days respectively.The colon histological score was made according to Dieleman＇s method,and the expression of ICC and SCF in distal colon was detected by immunohischemistry and Western blot analysis. Results：Comparing with normal mice,the colon histological score showed a peak level at 7^th day （P 〈 0.05）. The number of distal colon ICC in UC groups was more significantly decreased than that in the normal mice（P 〈 0.05）. Meanwhile,the expression of SCF of distal colon in UC groups was significantly decreased in UC groups compared with that in normal mice（P 〈 0.01）. There were high positive correlations between SCF and ICC （UC 4 d,r = 0.862,P = 0.027;UC 7 d,r = 0.948,P = 0.004）. Conclusion：The decrease of SCF and ICC＇in the distal colon of mice with ulcerative colitis may be an effective factor which is associated with the mechanism of ulcerative colitis.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第5期648-651,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省自然科学基金资助(BK2008466)
