摘要
通过蛋白质双向电泳和质谱技术,对低温前后抗寒品种东农冬麦1号和非抗寒品种济麦22的蛋白质组进行了比较分析.结果表明：在pH 4-7范围内,在东农冬麦1号和济麦22分蘖节蛋白表达图谱中发现55个蛋白点在低温胁迫后的表达量与低温前有明显差异,其中47个点在两个品种中均有表达.低温处理后,有23个蛋白点的丰度值变大,东农冬麦1号的绝对丰度高于济麦22;7个蛋白点的丰度值变小,东农冬麦1号的绝对丰度低于济麦22;东农冬麦1号特有8个蛋白点表达量上升.所有上调的蛋白点中东农冬麦1号的表达量是济麦22的2.1-16.5倍,超过4倍以上的点有10个;所有下调的点中东农冬麦1号的表达量是济麦22的0.1-0.4倍.对所有的差异蛋白点进行质谱分析,并通过数据库检索进行蛋白质鉴定,得到了其中51个蛋白的完整肽指纹图谱,这些蛋白中逆境蛋白占15.7%、代谢相关蛋白占27.5%、信号分子占19.6%、未知蛋白占9.8%、其他类蛋白占27.4%.这些蛋白的表达可能对东农冬麦1号抗寒性具有重要作用.
By using two-dimensional electrophoresis and mass spectrometry techniques, the proteomes in the tillering node of cold-resistant winter wheat Dongnongdongmai 1 and cold-susceptible winter wheat Jimai 22 before and after low temperature treatment were comparatively analyzed. It was observed that within the range of pH 4-7, there was a significant difference in the expression level of 55 protein spots in the protein profiles of tiilering node before and after low temperature treatment, and 47 of the 55 protein spots were expressed in the two cuhivars. After low temperature treatment, the abundance value of 23 protein spots increased, with the absolute abundance being higher in Dongnongdongmai 1 than in Jimai 22, while that of 7 protein spots decreased, with the absolute abundance being lower in Dongnongdongmai 1 than in Jimai 22. The expression level of 8 protein spots specific in Dongnongdongmai 1 was up-regulated. In all of the up-regulated proteins, their expression level in Dongnongdongmai 1 was 2. 1 - 16. 5 times as high as that in Jimai 22, among which, 10 protein spots had an expression level of 4 times high. In the down-regulated proteins, their expression level in Dongnongdongmai 1 was 0. 1-0. 4 times as high as that in Jimai 22. The differential protein spots were detected by mass spectrometry, and identified by compared with retrieval database. A total of 51 protein spots fingerprints were obtained, among which, 15.7% were of stress protein, 27.5% were of metabolism-related protein, 19.6% were signaling molecules, 9. 8% were of unknown protein, and other proteins took up 27.4%. These variously expressed proteins might play an important role in the cold resistance of Dongnongdongmai 1.
出处
《应用生态学报》
CAS
CSCD
北大核心
2009年第5期1092-1098,共7页
Chinese Journal of Applied Ecology
基金
东北农业大学创新团队项目(C×2003)
东北农业大学博士研究基金项目资助.
关键词
冬小麦
抗寒性
蛋白质组
双向电泳
质谱分析
winter wheat
cold resistance
proteome
two-dimensional electrophoresis
mass spectrometry
