摘要
在鱼类中首次应用地高辛标记的特异引物原位ＤＮＡ合成技术，将Ｐｘ基因Ｅ３外显子定位于黄鳝粗线期８号二价体的８ｑ１５～ｑ２６区间和ｌ号二价体的ｌｑ３２～ｑ３７区间，证实了异种生物基因探针和引物原位ＤＮＡ合成技术用于基因定位研究的可行性。
s Peroxidase ( Px ) gene of rice-field eels ( Monopterus albus ) was successfully localized to the segmenta of two bivalents, 8ql5～q26 and 11q32～q37 with digoxigenin labeled primed in situ labeling (PRINS) techinque, which is utilized for localization of single copy gene of fish for the first time. Our results demonstrated the possibility of mapping genes in fish by the DNA probes of other spocies and PRINS technique.
基金
国家自然科学基金
关键词
外显子
引物原位
DNA合成
基因定位
黄鳝
二价体
Rice-field eels, Bivalents, Gene mapping, Peroxidase ( Px ), Primed in situ labeling
