摘要
４个马铃薯栽培种和４个野生种叶肉原生质体黑暗中进行紫外线辐射处理。观察了不同剂量紫外线照射对原生质体分裂生长的影响。Ｓ．ｄｅｍｉｓｕｍ，Ｓ．ｔｕｂｅｒｏｓｕｍ，Ｓ．ｂｕｌｂｏｃａｓｔｕｍ，Ｓ．ｐｈｕｒｅｊａ和Ｓ．ｂｒｅｖｉｄｅｎｓ最低失活剂量分别为８ｍｉｎ，５．５ｍｉｎ，４．０ｍｉｎ，２．０ｍｉｎ和１５ｍｉｎ。３种紫外线照射方式中，“１５ｗ，６０ｃｍ”照射方式失活效果最好。刚分离的原生质体对紫外线最敏感，随着原生质体培养进程，其紫外线抗性逐渐增强。基因型、倍性水平、原生质体体积对原生质体紫外线失活剂量有影响。
Mesophyll protoplasts from 4 potato cultivars and 4 wild species were irradiated by ultraviolet (UV) in the dark.The influences of UV irradiation on the division and growth of the protoplasts were investigated.The minimum UV irradiation dosages (MUI) of S.demissum,S.tuberosum,S.bulbocastanum,S.phureja and S.brevidens were 8min,5.5min,4.0min,2.0min and 1 5min, respectively.Among the three ways of UV irradiation,“15W,60cm” showed the most effective inactivation.Freshly isolated protoplasts were most sensitive to UV irradiation,after that their sensitivities decreased gradually.The UV inactivation dosage could be affected by ploid level,genotype,volume of protoplast.
出处
《生物工程学报》
CAS
CSCD
北大核心
1998年第1期58-63,共6页
Chinese Journal of Biotechnology
基金
国家"八五"攻关
关键词
马铃薯
叶肉
原生质体
紫外辐射效应
Potato cultivars and wild species,mesophyll protoplast,UV irradiation
