摘要
小麦D2型细胞质与特定的核结合,对长光照(≥15小时)反应敏感,表现为雄蕊雌化,花粉败育。本实验通过扫描电镜观察了雌化的雄蕊横切面,发现有类似于胚珠的结构,但没有胚珠组织,也没有花药壁和花粉粒的结构。同时应用单向SDS-PAGE技术,对(C)-N26(D2型细胞质)小麦和N26(B型细胞质,核基因组相同)小麦的细胞质可溶性蛋白、叶绿体可溶性蛋白、线粒体可溶性蛋白多肽进行了比较分析,结果表明:在短光照条件下(≤14.5小时)(C)-N26小麦和长光照条件的N26小麦细胞质可溶性蛋白、线粒体可溶性蛋白和叶绿体可溶性蛋白多肽的SDS-PAGE图谱无差异。而(C)-N26小麦在长光照和短光照条件下SDS-PAGE图谱存在明显的差异。这种差异总趋势是长光照条件下蛋白多肽减少。故推测小麦D2型光敏性细胞质雄性不育,在长光照条件下,雄蕊雌化与不育表型的表达可能与核基因、线粒体基因和叶绿体基因的表达阻遏调控有关。
The combination of D 2 type cytoplasm and certain nucleus can cause wheat to be sensitive to long-day condition(≥15 hours)with the expression of pistillody of stamens and pollen abortion. Observation of tranverse sections using the scanning eletron microscope revealed that pistillate stamens contained ovule-like structures but had no ovule tissue、 tapetal cells and pollen grains. Comparison was made using unidirectional SDS-PAGE technique among soluble proteins of chloroplast、 mitochondria and cytoplasm derived from wheat (c)-N26(D 2type cytoplasm) and wheat N26(B type cytoplasm, the same nucleric genome). The SDS-PAGE maps of soluble proteins of chlorplast、 mitochondria and cytoplasm derived from (c)-N26 under a short-day condition(≤14.5hours)showed no difference to that of N26 under a long-day condition.But there were distinct differences between SDS-PAGE maps of (c)-N26 cultured on the long-day condition and short-day condition respectively. The trends is the reduction of proteins. So it can be infered from the facts above that on the long-day condition pistillody of stamens in D 2 PCMS line is probably relation to repression of genes expression in nuclei、chloroplast and mitochondria.
出处
《植物研究》
CAS
CSCD
北大核心
1998年第1期68-72,共5页
Bulletin of Botanical Research