摘要
目的:研究金针菇多糖(FVP)对小鼠免疫细胞产生肿瘤坏死因子-α(TNF-α)、干扰素-γ(INF-γ)、白细胞介素-2(IL-2)的影响。方法:MTT比色法检测细胞代谢活力,ELISA方法检测TNF-α、INF-γ、IL-2的含量。结果:FVP(200、100、50μg/mL)可增强小鼠脾细胞及腹腔渗出细胞代谢MTT的活力,促进小鼠脾细胞分泌TNF-α、IFN-γ、IL-2,促进小鼠腹腔渗出细胞分泌TNF-α,并且以促进TNF-α分泌作用最为明显;FVP(100、50、25 mg/kg)可以提高荷S180瘤小鼠血清TNF-α、IFN-γ的含量。结论:FVP可能通过促进小鼠免疫细胞产生TNF-α、IFN-γ、IL-2而发挥免疫调节功能。
Objective ：To study the effect of Flammulina velutipes polysaccharides （FVP） on the production of tumor necrosis factorα （TNF-α）,interferonγ（ INF-γ） and interleukin 2 （IL-2） by murine immunocytes. Methods：The cell＇s metabolic activity was determined with methylthiazolyl tetrazolium（MTT） colorimetry assay and the amounts of TNF-α ,INF-γ and IL-2 were measured by ELISA. Results：FVP （200,100,50 μg/mL）could promote the metabolic activity of murine splenocytes and peritoneal exudate cells（PEC） and increase the amounts of TNF-α, INF-γ and IL-2 in the supernatants of splenocyte cultures,and the amount of TNF-α in PEC cultures, with the most marked increase on TNF-α level. FVP（ 100,50,25 mg/kg）could raise the serum levels of TNF-α and INF-γ in S180 tumor-bearing mice. Conclusion：FVP may regulate murine immune function through promoting the production of TNF-α, INF-γ and IL-2.
出处
《中药材》
CAS
CSCD
北大核心
2009年第4期561-563,共3页
Journal of Chinese Medicinal Materials
基金
广东省自然科学基金(7005189)
