摘要
目的观察人脐血基质细胞及骨髓瘤患者骨髓基质细胞对骨髓瘤KM3细胞生物学特性的作用。方法体外分离培养骨髓瘤骨髓基质细胞和人脐血源基质细胞,与骨髓瘤KM3细胞共培养。采用扫描电镜观察共培养后基质细胞和KM3细胞的位相关系;应用CCK-8、PI染色流式细胞仪和AnnexinV/PI双标法检测不同培养条件下KM3细胞的增殖、细胞周期和凋亡率。结果人脐血基质细胞比骨髓瘤患者骨髓基质细胞有更强的抑制KM细胞增殖的作用;共培养后骨髓瘤患者骨髓基质细胞使KM3细胞阻滞于G0/G1期[(59.70±1.28)%],人脐血基质细胞使S期的KM3细胞比例增高[(46.07±2.46)%];KM3/MM-BMSCs组KM3细胞凋亡率为(3.26±0.12)%明显低于KM3/hUCBDSCs组KM3细胞凋亡率(4.76±0.12)%(P<0.01);KM3/MM-BMSCs组KM3细胞BaxmRNA的表达较KM3/hUCBDSCs组明显下降(P<0.01);KM3/hUCBDSCs组KM3细胞Bcl-2mRNA的表达较KM3细胞悬浮培养组下降(P<0.05),KM3/MM-BMSCs组KM3细胞Bcl-2mRNA的表达则明显升高,为悬浮培养组的1.982倍(P<0.01)。结论骨髓瘤基质细胞微环境抑制骨髓瘤细胞凋亡和死亡;而人脐血源基质细胞有更强的抑制骨髓瘤细胞增殖的作用,并促进其凋亡。
Objective To observe the effect of the human umbilical cord blood-derived stromal cells （hUCBDSCs） and multiple myeloma bone marrow stromal cells （MM BMSCs） on the biological characteristics of KM3 cells. Methods After KM3 cells were co-cultured with hUCBDSCs or MM BMSCs, the proliferation, cell cycle and the apoptotic rate were determined by growth curve plotting and flow eytometry respectively. Realtime Q-PCR was used to measure the mRNA expressions of Bcl-2 and Bax. Results As compared with MM BMSCs, hUCBDSCs markedly suppressed the proliferation of the KM3 cells. KM3 ceils in the KM3/MM-BMSCs co-culture group were arrested in G0/G1 phrase , while KM3 cells in the KM3/hUCBDSCs co-culture group predominantly in S phrase. The apoptotie rate of KM3 cells in the KM3/hUCBDSC co-culture group were higher than that in the KM3/MM-BMSCs co-culture group. Conclusion In comparison with MM BMSCs, hUCBDSCs inhibit the proliferation of KM3 cells and promote the apoptosis.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2009年第10期879-882,共4页
Journal of Third Military Medical University
基金
国家自然科学基金(30670890)
重庆市医学重点学科建设基金(2006C028)~~
关键词
多发性骨髓瘤
人脐血基质细胞
骨髓基质细胞
增殖
凋亡
multiple myeloma
human umbilical cord blood-derived stromal cells
bone marrow stromal cells
proliferation
apoptosis
