摘要
目的建立陇西产黄芪药材HPLC-DAD-ELSD指纹图谱,为全面科学地控制药材质量提供方法。方法采用HPLC-DAD-ELSD串联技术,流动相A:10%乙腈,B:90%乙腈;检测波长:265 nm;体积流量:1 mL/min,柱温:35℃;进样量:20μL;增益值:20;漂移管温度:55℃;加热器级别:65%;气体压力;2.068 5×105Pa。以10批甘肃陇西不同产地的黄芪药材建立黄酮类和皂苷类化合物指纹图谱共有模式,并采用"中药色谱指纹图谱相似度评价系统"软件进行数据处理。结果建立的方法对黄芪药材中黄酮类成分、皂苷类成分均有良好的分离,一次进样同时测定了两类不同成分的指纹图谱,不同批次药材间的相似度符合相关规定。结论该方法可用于黄芪中黄酮类成分和皂苷类成分指纹图谱的同时测定,控制药材质量。
Objective To establish the HPI.C-DAD-ELSD fingerprint of Radix astragali, provide new methods for science quality control of the medicinal materials. Methods Application of HPLC-DAD-ELSD techniques were connected in series. The mobile phase A： 10% acetonitrile, B： 90% acetonitrile, detecting wavelength： 265 nm, flow rate： 1 mL/min, column temperature： 35 C, sample size： 20 μL, gain： 20, tube： 55 ℃, neb： 65%, air pressure： 2. 068 5×10^5; Pa. The mutual mode was established depending on ten Astragalus samples from different growing areas in Gansu. The software ＂Similarity Evaluation System for Chromatographic Fingerfrint of Chinese Materia Medica＂ was applied to analyzing. Results The established method is good for the separation of saponins, flavonoids from Radiz Astragali, and simultaneous determination of the two different components in one sample injection. The similarity ofdiffcrent hatches of medicinal materials ix fit for the requirement. Conclusion The method is workable to simultaneously determine saponins and flavonoids fingerprint from Radix Astragali, and to control its quality.
出处
《中草药》
CAS
CSCD
北大核心
2009年第5期804-806,共3页
Chinese Traditional and Herbal Drugs
基金
天津市科技创新专项资金项目(06FZZDSH00408)
