摘要
目的用稳定性检索标记的１５Ｎ－甘氨酸和１３Ｃ－亮氨酸双示踪技术研究肝炎后肝硬化病人血浆氨基酸和整体蛋白质更新速率。方法１１例正常人和１９例肝硬化加入（１２例代偿期，７例失代偿期）恒速静滴６小时示踪剂后测定血浆中标记氨基酸水平，并计算血浆中氨基酸和整体蛋白质的更新速率，合成速率和分解速率。结果肝硬化时血浆氨基酸和蛋白顶的更新速率，合成和分解速率均明显加快，分解速率大于合成速率，此现象在失代偿期尤为明显。结论肝硬化时机体氨基酸和蛋白质代谢处于高流量状态，分解速率明显高于合成速率，处于负氮平衡状态。
Objective Stable isotopic labled tracers 15N-Gly and 13C-Leu were used for investigation on changes of plasma amino acids and whole-body protein turnover rates in post-viral hepatic cirrhotics. Methods Stable iostopic tracers were infused in constant rate into 11 normal subjects and 19 cirrhotics (12 compensated, 7 decompensated) for 6 hours. The concentration of labled amino acids were measured and protein metabolic parameters overall turnover rates, synthetic and catabolic rates were calculated. Results Marked increasing of whole-body protein turnover rates, synthetic and catabolic rates were found in all cirrhotics, and the increasing of catabolic rate in cirrhosis was much greater than that of synthetic rate. This phenomenon was more obvious in the decompensated cirrhotics. Conclusion The metabolism of amino acids and protein in cirrhosis was characterized by status of high turnover rates, the catabolic rate was greater than synthetic rate, and the patients were at a state of negative nitrogen balance. [
出处
《中华消化杂志》
CAS
CSCD
北大核心
1998年第1期28-30,共3页
Chinese Journal of Digestion
关键词
肝硬变
蛋白质
氨基酸
更新速度
肝炎
Cirrhotics Protein Amino acids Turnover rate Stable isotope
