摘要
目的观察不同复温速率及浅低温对兔肢体爆炸伤合并海水浸泡后机体炎症反应的特点。方法复制肢体爆炸伤合并海水浸泡致低体温[（31．0±0．5℃）]模型。成年家兔24只，随机分为4组，每组6只。Ⅰ组复温至（38．0±0．5）℃，复温速率（8．94±0．93）℃／h；II组复温至（38．0±0．5）℃，复温速率（3．88±0．22）℃／h；III组复温至（38．0±0．5）℃，复温速率（2．18±0．12）℃／h；H组复温至（34～35）℃并维持至实验结束，复温速率（4．49±0．66）℃／h。以调节环境温度及加温输液的方法将体温恢复到目标体温后维持该体温观察6h。于致伤前（To）、浸泡降温后（T1）、复温即刻（T2）、复温后3h（T3）、复温后6h（T4）共5个时相点检测血清肿瘤坏死因子（TNF-α）、白介素-1β（IL-1β）、白介素-6（IL-6）。实验结束后取动物心、肝、肠、肺、肾组织，测定组织匀浆髓过氧化物酶（MPO）活性。结果复温后，I组、H组IL-1β、IL-6、TNF-α值较Ⅱ组、Ⅲ组明显升高（P〈0．01或P〈0．05），其中I组升高更为显著。I组、H组心、肝、肠、肺、肾组织匀浆中，MPO活性较Ⅱ组、Ⅲ组明显增高（P〈0．0l或P〈0．05），Ⅱ组、Ⅲ组比较差异无统计学意义。结论肢体爆炸伤合并海水浸泡致低体温后，快速复温及维持机体低体温均可导致机体内IL-1β、IL-6、TNF-α水平明显升高，组织中MPO活性明显增高；缓慢复温则可以明显抑制这3种炎症因子的水平及组织中MPO活性。
Objective To investigate effects of different rewarming rates and maintenance of light hypothermia on inflammatory response in rabbits after limb blast injury, coupled with seawater immersion. Methods First, the model of limb blast injury coupled with seawater immersion was reproduced [ the animals were immersed to low body temperature of （31 ± 0.5 ） ℃ ]. Then, 24 adult rabbits were randomly divided into group I [the rapid rewanning group, n =6, rewarmed to （38 ±0.5）℃ at a rate of （8.94 ±0. 93）℃/h], group U [the slow rewarming group, n =6, rewarmed to （38 ±0.5）℃ at a rate of （3.88 ±0.22）℃/h], group Ⅲ [ another slow rewarming group, n = 6, rewarmed to （38 ± 0.5 ）℃ at a rate of （2.18 ± 0.12）℃/h] , and the H group [ the hypothermia group, rL = 6, rewarmed to （34 -35 ）℃ at a rate of （4.49 ± 0.66 ）℃/h and kept at that temperature till termination of the experiment ]. Regulation of ambient temperature and warm transfusion were used to restore body temperature to target levels and maintained there for 6 hours. Blood samples were taken at 5 different times, i.e. pre-injury time（T0）, post-immersion time （T1 ）, the time when rewarming started （ T2 ）, 3 h after rewarming （ T3 ）, and 6 h after rewarming （ T4 ）. Tissue samples from heart, liver, intestinum, lung and kidney were also collected. Levels of TNF-α （tumor necrosis factor-or） , IL-1β （interleukin-1β） and IL-6 （interleukin-6） in plasma and MPO （myeloperoxidase） in homogenate were detected. Results Following rewarming, TNF-α, IL-1β, IL-6 concentrations in the plasma of the animals in group I and group H were significantly higher when compared with those of the animals in group Ⅱ and group Ⅲ （ P 〈 0.05, P 〈 0.01 ）, and MPO activity in homogenate was significantly higher when compared with that of the animals in group Ⅱ and group Ⅲ （ P 〈 0.01, P 〈 0. 05 ）, and no statistical difference could be seen between group Ⅱ and Ⅲ （ P 〉 o. 05 �
出处
《中华航海医学与高气压医学杂志》
CAS
CSCD
2009年第2期67-70,共4页
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
基金
基金项目：全军“十一五”指令性课题（06D004）
关键词
复温速率
海水浸泡
低温
炎症反应
Rewarming rate
Seawater immersion
Hypothermia
Inflammatory response
