摘要
目的探讨来氟米特对MRL/lpr狼疮小鼠肾脏JAK/STAT1信号转导途径的影响。方法14只12周龄雌性MRL/lpr狼疮小鼠随机分为空白对照组和治疗组(来氟米特35 mg.kg-1.d-1×8周)。检测小鼠肾脏磷酸化STAT1(p-STAT1)蛋白及细胞信号传导抑制因子(SOCS)-1 mRNA表达情况,并观察小鼠体重、24-h尿蛋白、血浆抗双链DNA(ds-DNA)抗体浓度、肾组织病理改变及肾小球免疫荧光变化。结果治疗组小鼠24-h尿蛋白及血浆抗ds-DNA抗体浓度均明显低于对照组(P<0.01),肾脏p-STAT1蛋白及SOCS-1 mRNA表达均低于对照组(P<0.01);肾组织病理明显改善。结论来氟米特能抑制肾脏JAK/STAT1信号转导途径,对MRL/lpr狼疮小鼠有治疗作用。
Objective To explore the influence of leflunomide on Janus protein-tyrosine kinase（JAK）/signal transducer and activator of transcription（STAT） signal transduction pathway in renal tissue of MRL/Ipr lupus mice. Methods Fourteen MRL/1pr mice were divided into control grouo and leflunomide group（35mg·kg^-1·d^-1 8 weeks）. The expressions of phosphorylation of STAT1（p-STAT1） protein and SOCS-1 mRNA in renal tissue were detected and the changes in proteinuria,antibody of ds-DNA, histopathological variations and the deposition of immune eomplex（IC） in renal glomerule were observed.Results The amount of urinary protein and antibody of ds-DNA in leflunomide group were significantly lower than those in control group. The expressions of p-STAT1 protein and SOCS-1 mRNA in leflunomide group were significantly lower than those in control group.The histopathological changes were improved remarkably in leflunomide group. Conclusion Leflunomide can inhibit the expression of p-STAT1 and prevents the excess activation of JAK/STAT1 signal transduction pathway, which may be one of the mechanisms for the therapeutic effects in the MRL/1pr lupus mice.
出处
《江苏医药》
CAS
CSCD
北大核心
2009年第5期561-563,621,共4页
Jiangsu Medical Journal
