摘要
探讨Src激酶抑制剂FB2抗前列腺癌的作用及机制.整体动物实验观察FB2对人前列腺癌PC-3细胞在裸鼠异体中生长的影响;MMT、克隆原形成、细胞黏附试验观察FB2对肿瘤细胞增殖、黏附的影响;流式细胞术分析FB2对细胞周期的影响;Western blot观察细胞及组织内Src及p-Src表达.实验结果表明FB2抑制PC-3细胞裸鼠移植瘤的生长;FB2抑制PC-3细胞增殖及对基底膜成分的黏附,并将细胞周期阻滞于G1期;机制研究表明,FB2抑制PC-3细胞及瘤组织中Src激酶Tyr416磷酸化.因此Src激酶抑制剂FB2在体内外均有较强的抗前列腺癌作用,其机制可能与抑制Src激酶Tyr416磷酸化有关.
Aim at evaluate the anticancer activity of FB2, a Src kinase inhibitor, on prostate cancer. Animal experiments are used to determine the antitumor activity of FB2 on PC -3 xenograft tumor growth in vivo. MTT assays, colony formation and adhesion assays are utilized to assess impact of FB2 on the cell proliferation and cell adhesion. Flow cytometry is used to analysis cell cycle distribution. Western blot is used to analysis the level of phosphorylation of Src at Tyr 416 and Src. FB2 had significant antitumor activity on PC-3 xenograft in nude mice and inhibited the cell proliferation. FB2 inhibited cell adhesion and caused cell cycle arrest in G1 phase. FB2 inhibited Src phosphorylation at Tyr 416. FB2 had inhibitory effects on prostate cancer in vitro and in vivo through blocking Src phosphorylation at Tyr 416.
出处
《哈尔滨商业大学学报（自然科学版）》
CAS
2009年第2期129-134,共6页
Journal of Harbin University of Commerce：Natural Sciences Edition
