摘要
以典型的顽拗性种子黄皮（Ｃｌａｕｓｅｎａｌａｎｓｉｕｍ）为实验材料，运用３５Ｓ－Ｍｅｔ标记黄皮种子发育期间和脱水胁迫时合成的蛋白质．比较合成蛋白的二维电泳图谱，发现顽拗性种子黄皮胚轴在整个发育期间保持基本一致的蛋白质合成模式，只有在花后６３ｄ发育后期胚轴中能产生一种相对分子质量为２．６×１０４的新蛋白．根据实验结果，解释了顽拗性种子的一些生理特性，提出了顽拗性种子不耐脱水的另一种成因．
Wampee( Clausena lansium ),a typical recalcitrant seed, was chosen as experimental material, and the protein synthesis in seed axes during development and under drying stress was labelled by 35 S Met. From the two dimentional electrophoresis maps of synthesized proteins,it was found that wampee seed axes had almost same kind of protein synthesis pattern through the whole development period except that a new 2 6×10 4 protein appeared at 63 days after flower, and the 2 6×10 4 protein was also induced by artificial drying stress at 53 and 63 days after flower.The stress responding ability of wampee to drying was weaker than orthodox seeds According to the results, the explanation of some specific physiology behavior of recalcitrant seed was made and another cause why recalcitrant seeds were desiccation sensitive was proposed
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
1998年第3期128-130,共3页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家自然科学基金
广东省自然科学基金
关键词
黄皮
顽拗性种子
蛋白合成
种子发育
萌动
Clausena lansium , recalcitrant seed, desiccation sensitivity, protein synthesis
