摘要
采用PCR-SSCP技术分析了重庆山地黄牛及其杂交牛CAST基因的多态性。在所做的2对引物中研究表明：在第18外显子处没有发现多态性；在第9外显子处发现多态性，在重庆山地黄牛及其杂交牛群体中都有AA、AB、BB3种基因型。在西杂牛（重庆山地黄牛X西门塔尔牛）、红杂牛（重庆山地黄牛X红安格斯）中A为优势基因，在重庆山地黄牛中B为优势基因。通过对各遗传多态性指标分析发现，重庆山地黄牛、西杂牛、红杂牛处于平衡状态，几个品种牛踟均在0．250～0．500，处于中度多态；几个品种牛的杂合度都偏高。
The polymorphism of CAST gene in Chongqing mountain cattle and its hybrids was analyzed by PCR-SSCP. In two pairs of primers, there were no polymorphism detected by the 18^th exon of the bovine CAST and there were three genotypes （AA, AB and BB） detected by the 9th exon of the bovine CAST in all cattle. The frequeney of A allele was higher than that of B allele in Xiza cattle （Chongqing cattle x Simmental）and Hongza （Chongqing cattle ×Angus） cattle breeds,in Chongqing cattle, the frequency of B allele was higher than A allele.All three breeds were at Hardy-Weinberg equilibrium. In Chongqing cattle, Xiza cattle, Hongza cattle, the polymorphisms information contents were moderate polymorphisms（0.250 〈 PIC 〈 0.500）.
出处
《安徽农业科学》
CAS
北大核心
2009年第13期5892-5893,5911,共3页
Journal of Anhui Agricultural Sciences
基金
重庆市科技攻关计划项目(2006AC1042)
