摘要
目的:探讨冠状动脉粥样硬化斑块不同区域肥大细胞的分布及与新生血管的关系。方法:将经苏木素-伊红染色筛选出的属于中晚期的动脉粥样硬化病变(脂质核心面积大于斑块面积的40%)的组织块和10例尸检正常冠状动脉标本进行抗肥大细胞类胰蛋白酶和CD31单克隆抗体免疫组化染色。结果:在正常内膜、斑块的纤维帽区、核心区、肩区肥大细胞密度(细胞数/视野)和新生微血管密度(微血管数/视野)分别为1.2±1.7、2.6±2.1、3.1±2.4、7.2±3.9和0、2.8±1.6、3.6±1.8、8.3±2.6,斑块肩区肥大细胞密度(MCD)和新生微血管密度(MVD)明显高于其它部位(P(0.05或P(0.01)且二者存在明显的正相关(r=0.545;P(0.05)。结论:冠状动脉粥样硬化斑块中肥大细胞的分布与新生微血管密度具有相关性且可能影响斑块的稳定性。
Objective： To study the distribution of mast cells in atherosclerotic lesions of human coronary arteries , and to investigate the relationship between mast cell density （MCD） and microvessel density（MVD）. Methods ：42 samples with coronary atherosclerosis and 10 autopsies of normal coronary arteries specimens were obtained. Plaque morphology was evaluated by using hematoxylin and eosin stained slides. Then,tissue blocks with large extracellular lipid core （more than 40% of the Plaque area）were needed and all were stained with monoclonal antibodies against the tryptase of mast cells and CD31 of endothelial cell. Results ：The density（ cells/mm2 ）of mast cells in shoulder region of plaque（7.2±3.9） was much higher than that in normal intima,cap and core region（ 1.2±1.7,2.6± 2.1,3.1±2.4）, and the density （microvessels/mm2） of the microvessel in shoulder region of plaque （ 8.3± 2.6 ） was also much higher than that in normal intima, cap and core region（0, 3.6±1.8, 2.8±1.6）. A close positive correlation was found between MCD and MVD （ r = 0.545, P 〈 0.05 ）. Conclusion ： Mast cells play a role in the angiogenesis of atherosclerotic plaque , and they may par- ticipate in the destabilization of plaque.
出处
《牡丹江医学院学报》
2009年第2期1-3,共3页
Journal of Mudanjiang Medical University
基金
牡丹江医学院重点项目(D2006-12)
