摘要
目的：对FZ06008株、FZ17408株VP1进行基因特性分析。方法：采集福州市2008年6月手足口病人的标本,接种于RD细胞进行病毒分离,采用RT-PCR方法进行核酸鉴定,将VP1基因克隆和序列测定,并利用DNA-STAR软件对VP1基因进行遗传性分析。结果：与标准株B rCr相比,FZ06008和FZ17408的VP1编码区没有出现核苷酸的缺失和插入,氨基酸同源性约为94.6%,核苷酸同源性约为79.1%;与安徽省2008年流行株703813-Fuyang-08和我国深圳2003年流行的SHZH03株相比,氨基酸同源性约为99%-99.3%。结论：FZ06008株和FZ17408株有可能来源于2003年深圳地区EV71大规模流行时的毒株,其进化途径可能与安徽阜阳的标准株703813-Fuyang-08相近。
Objective：To analyze phylogenic tree of FZ06008 and FZ17408 based on the VP1 gene sequences. Methods：Specimens from patients with HFMD symptom in Fuzhou during June 2008 were collected and inoculated for RD cell. They were detected by a reverse transcription polymerase chain reaction （ RT - PCR）. And VP1 gene was cloned and sequenced, then the phylogenic tree was completed by DNASTAR software. Result：Compared with BrCr, FZ06008 and FZ17408 were without nucleotide absence asld insert in VP1 gene, and amino acid homology was about 94. 6% , and the nucleotide homology was about 79. 1%. Among FZ06008, FZ17408, SHZH03 and 703813 - Fuyang - 08 strains, amino acid homology was about 99 % - 99. 3 %. Conclusion： FZ06008 and FZ17408 may have the same genotype with Shenzhen predominant strains in 2003, and its evolution path is similar to 703813 - Fuyang -08 strain.
出处
《中国卫生检验杂志》
CAS
2009年第4期749-751,共3页
Chinese Journal of Health Laboratory Technology
