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牛朊蛋白在CHO/dhfr-细胞中的稳定表达 被引量：4

Stable Expression of Bovine Prion Protein in CHO/dhfr-cells
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摘要 从T载体上得到的目的基因和dhfr共扩增基因真核表达载体构建了pCI-PrP102。纯化后的重组质粒通过脂质体转染CHO/dhfr-细胞,经MTX加压筛选获得稳定表达的细胞株,间接免疫荧光试验检测到目的蛋白的表达。 ：The target genes from T - vector were connected with dhfr co - amplified gene of eukaryotic vector and constructed pCI -PrP102. Purified recombinant plasmids were transferred into CHO/dhfr- cell through liposome, and obtained the stable expression of the cell strains after MTX screening, and indirect immunity fluorescence analysis detected the expression of the goal protein.
作者 丁耀忠 张杰 刘永生 陈豪泰 曾巧英 马丽娜 马艳平 杨生海 殷宏
机构地区 甘肃农业大学 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室
出处 《江西农业学报》 CAS 2009年第4期101-103,共3页 Acta Agriculturae Jiangxi
基金 国家自然科学基金项目(30671563)
关键词 牛朊蛋白 二氢叶酸还原酶缺陷型CHO细胞 稳定表达 Bovine prion protein CHO/dhfr-cell Stable expression
分类号 S823.2 [农业科学—畜牧学]
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参考文献10

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二级参考文献5

共引文献2

同被引文献24

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江西农业学报
2009年 第4期

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