摘要
以‘明水’梨(Akemizu)叶片为外植体,研究了不同基本培养基,不同浓度噻重氮苯基脲(TDZ)和吲哚丁酸(IBA)的组合,不同暗培养时间以及不同浓度的硝酸银(AgNO3)对梨叶片不定芽再生的影响。试验结果表明,在NN69+TDZ 1.5mg/L+IBA0.2 mg/L+AgNO31.0 mg/L中暗培养25 d后,叶片不定芽再生率最高,达到71.8%。
Using the isolated Leaves of ‘Akemizu＇ pear as the explants, the regeneration system of the adventitious bud from the leaves was studied, and the influences of different basal media, combinations of TDZ and IBA with different concentrations, cultivation time in the dark and AgNO3 concentration on the regeneration of adventitious bud were explored. The results showed that when the leaves were cultivated on the medium NN69 ＋ TDZ 1.5 mg/L ＋ IBA 0.2 mg/L ＋ AgNO3 1.0 mg/L for 25 days under dark condition, the regeneration ratio of the adventitious bud was the highest, reaching to 71.8%.
出处
《江西农业学报》
CAS
2009年第4期42-44,47,共4页
Acta Agriculturae Jiangxi
基金
江苏省高技术研究项目(BG2006313)
关键词
明水梨
不定芽
再生
培养基
Pyrus pyrifolia Nakai ‘Akemizu＇
Adventitious bud
Regeneration
Medium
