摘要
为探讨植物储存蛋白在植物适应环境中的作用,本研究利用2-D和质谱技术从中国南部3省5个红树群落来源的木榄叶片总可溶性蛋白中鉴定到6种主要储存蛋白(181、182、188、194、198和204,相对分子质量约为33000～34000,等电点介于5.5～6.45之间)以及2种营养型储存蛋白(266和274,相对分子质量约为23500,等电点分别为5.1和5.3)。进一步的相关性分析结果显示:在上述几种植物储存蛋白中,182、198和204含量的变化与环境中的年均气温之间具有明显的正相关性(P<0.05),而274则与环境中的土壤pH之间存在着明显的正相关性(P<0.05)。上述研究显示:在木榄叶片中,作为临时氮源的储存蛋白含量的变化可能与环境中的年均气温和土壤pH之间具有直接的相关性。
To reveal the action of plant storage proteins in stress tolerance, 6 major storage proteins and 2 vegetative storage proteins were identified from the leaves of Bruguiera gymnorrhiza by 2-D and MS. The molecular weight of 181, 182, 188, 194, 198 and 204 is about 33000 to 34000Da, and their isoelectric point is about 5.5 to 6.45. While at the same time, the molecular weight of 266 and 274 is about 23500Da, and their isoelectric point is about 5.1 and 5.3, respectively. Further analysis showed that, among these storage proteins, 182, 198 and 204 were positively correlated with the mean annual temperature, while 274 was mainly correlated with the soil pH. So far, we suggest that as the temporary nitrogen sources, the contents of storage proteins in B. gyrnnorrhiza leaves are mainly affected by the mean annual temperature and soil pH.
出处
《药物生物技术》
CAS
CSCD
2009年第2期127-131,共5页
Pharmaceutical Biotechnology
基金
教育部高等学校科技创新工程重大项目培育资金项目(NO130105)
教育部"新世纪优秀人才支持计划"项目(NCET-05-0496)
关键词
木榄
蛋白质组
储存蛋白
环境因子
相关性
Bruguiera gymnorrhiza, Proteome, Storage protein, Environmental factor, Correlation
