摘要
目的:阐明细菌内毒素的主要致病成分脂多糖(LPS)对体外培养人真皮成纤维细胞增殖及转化生长因子-β1(TGF-β1)、γ-干扰素(IFN-γ)分泌的影响。方法:取正常及瘢痕皮肤行成纤维细胞培养后分为2个对照组及6个实验组。实验组分别与终浓度为0.005、0.01、0.05、0.1、0.5和1μg/mL大肠杆菌LPS(E.coli055:B5)培养,对照组为DMEM培养,并对刺激后细胞进行传代至表型稳定(第8代)。应用MTT比色分析法检测细胞增殖率的变化。分别于接种后8、24及48h收集培养细胞上清液,应用酶联免疫吸附测定分析法测定培养细胞上清TGF-β1、IFN-γ分泌量的变化。结果:①0.005～0.1μg/mLLPS组成纤维细胞增殖及TGF-β1的分泌增加、IFN-γ的分泌降低,随浓度增加作用增强,均在0.1μg/mL浓度点作用达高峰;②0.5及1μg/mLLPS组成纤维细胞增殖及TGF-β1的分泌下降,IFN-γ的分泌开始增加。③0.1μg/mLLPS组成纤维细胞增殖及TGF-β1、IFN-γ的分泌与阳性对照组比较,差异无统计学意义(P>0.05)。结论:一定浓度的LPS可能与增生性瘢痕形成有关。
Objective：To investigate the effect of LPS on the proliferation and excretion of transforming growth factor-β1 （TGF-β1 ） ,interferon-γ（IFN-γ） on human dermal fibroblast. Methods：Purified dermal fibroblasts derived from human normal skin were divided into 2 control groups and 6 test groups and exposed to LPS （ E. coli055 ： B5 ） of different concentrations （0. 005,0.01,0.05,0.1,0.5,1.0 μg/mL）. Cell proliferation was determined by MTT assay. The supernatant was collected 8,24 and 48 h after inoculation. TGF-β1 , IFN-γ were assayed by ELISA. Results：①LPS of lower concentrations （0. 005 -0.1 μg/mL） enhanced the proliferation and the excretion of TGF-β1 and prohibited the excretion of IFN-γ,and the effect showed a concentration dependent manner,the effect reached the peak when challenged with LPS in the concentration of 0.1 μg/mL. ②However,LPS of high concentration （0.5 and 1.0 μg/mL） showed opposite effect. ③When the concentration of LPS was 0.1 μg/mL, the collagen synthesis and the proliferation and the excretion of TGF-β1 , IFN-γ were similar to that of positive control group（ P 〉 0.05 ）. Conclusion ：LPS might play an important role in the formation of hypertrophic scar.
出处
《华北国防医药》
2009年第2期6-9,共4页
Medical Journal of Beijing Military Region
基金
国家自然科学基金资助项目(30371467)
关键词
脂多糖类
成纤维细胞
细胞增殖
转化生长因子-Β
Γ-干扰素
Lipopolysaccharides（LPS）
Fibroblast
Cell proliferation
Transforming growth factor beta
Interferon gamma
