摘要
目的:探讨凋亡抑制蛋白(IAP)家族中的新成员Livin在小细胞肺癌(SCLC)组织中的表达及其与细胞增殖、凋亡的关系。方法:采用RT-PCR技术检测SCLC组织中LivinmRNA的表达,并用蛋白质印迹法分析Livin蛋白的表达。应用流式细胞术检测小细胞肺癌细胞的增殖活性和凋亡水平。结果:30例SCLC组织中有19例Livin表达,阳性率为63.33%;而16例癌旁正常肺组织中Livin低表达,阳性率为12.5%,P<0.01。30例SCLC组织中,19例SCLC组织Livin表达阳性者,细胞增殖指数(PI)为(35.50±4.08)%;11例Livin表达阴性者,PI为(22.92±3.01)%,两者比较差异有统计学意义,P<0.01。19例SCLC组织Livin表达阳性者,肿瘤细胞早期细胞凋亡率为(2.77±0.34)%;11例Livin表达阴性者,肿瘤细胞早期细胞凋亡率为(4.04±0.51)%,两者比较差异有统计学意义,P<0.01。结论:Livin在SCLC组织中异常表达,有望成为SCLC诊断和基因治疗的新靶点。SCLC组织Livin基因表达与肿瘤细胞增殖和凋亡有关。
OBJECTIVE： To study the expression of Livin,a novel inhibitor of apoptosis protein（lAP） family member, in small cell lung cancer （SCLC） and its correlation with carcinoma cell proliferation and apoptosis. METHODS：The expression of Livin mRNA in SCLC tissues was measured by reverse transcription polymerase chain reaction （RT-PCR）. The expression of Livin protion was measured by Western blotting assay. Flow cytometry was used to detect proliferation and apoptosis. RESULTS： Livin was expressed in 19 of 30 SCLC （63.33%）. Livin expressed low levels in 16 para-cancerous lung tissues （12.5%, P^0. 01）. In 30 SCLC tissues,the average PI of 19 cases with positive Livin expression and 11 cases with negative expression were （35.50 ± 4. 080/00） and （22. 92 ± 3. 01）% respectively, with a significant deference, P%0.01. In 30 SCLC tissues, the apoptosis ratios of 19 cases with positive Livin expression and 11 cases with negvative expression were （2.77±0. 34）% and （4.04 ± 0.51）% respectively, with a significant deference, P〈0.01. CONCLUSION： Aberrant expression of Livin may be a new target for diagnosis and gene treatment of SCLC. The expression of Livin gene in SCLC tissues has a correlation with tumor cell prolif eration and apoptosis.
出处
《中华肿瘤防治杂志》
CAS
2009年第5期355-358,共4页
Chinese Journal of Cancer Prevention and Treatment
关键词
癌
小细胞肺
凋亡抑制蛋白
增殖
凋亡
carcinoma, small cell lung
inhibitor of apoptosis protein
proliferation
apoptosis
