摘要
目的:利用酶处理法制备猪骨材料,并对其生物相容性进行评价。方法:新鲜猪骨经深冻、脱脂、酶处理、冻干、辐照灭菌处理,检测其α-半乳糖抗原(α-Gal)的光密度值;观察其结构。观察猪骨浸提液对兔骨髓间充质干细胞(BMSCs)生长的影响;兔BMSCs与猪骨复合培养,扫描电镜观察。将猪骨植入Wistar大鼠皮下,组织学观察。结果:酶处理后猪骨的α-Gal光密度值显著低于新鲜猪骨组。猪骨松质呈三维网状多孔结构。猪骨浸提液对兔BMSCs的生长没有显著影响。复合培养1～3周,BMSCs与猪骨贴附紧密,细胞生长状态良好。皮下植入后,猪骨周围及腔隙内结缔组织炎性反应逐渐减轻,12周时与正常组织没有区别。结论:制备的猪骨移植材料去除了主要异种抗原,具有良好的生物相容性。
Objective： To develop a porcine bone graft through enzyme treatment and evaluate the biocompatibility of the graft produced thereby. Methods： Xenogeneic bone was prepared from porcine bone by a series treatment including deep-freeze, α-galactosidase treatment, defatting, freeze drying and irradiating, scanning electron microscope （SEM） was performed to observe the structure of the graft. The effect of the porcine bone on the growth of rabbit bone marrow mesenchymal stem cells （BMSCs） were investigated. The porcine bone and BMSCs were co cultured and SEM observation was performed to evaluate its affinity to cells. Porcine bone were subcutaneously implanted into Wistar rats and histological observation was performed. Results： The porous structure of prepared porcine cancellous bone was similar to that of human cancellous bone. The OD value of the enzyme treated porcine bone was significantly lower than that of fresh porcine bone. The porcine bone had no significant effect on the growth of rabbit BMSCs. Postoperative 1-3 weeks after co-culturing of porcine bone and BMSCs, the ceils adhered tightly to the surface of the porcine hone with a well status. Histological observation of subcutaneous im plantation showed that the inflammatory reaction reduced postoperative 1-12 weeks. Conclusion： The porcine bone prepared according to the methods contains good biocompatibility.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2009年第2期226-229,共4页
Chinese Journal of Anatomy
关键词
异种骨
制备
生物相容性
xenogeneic bone
preparation
biocompatiblity
