摘要
目的:研究透明质酸钠作为组织工程骨支架的可行性。方法:体外培养兔骨髓间充质干细胞(MSCs),在成骨诱导剂地塞米松等的诱导下,向成骨细胞转化,并使之与透明质酸钠凝胶复合,通过倒置相差显微镜和扫描电子显微镜观察细胞贴附情况。结果:地塞米松等诱导组细胞形态向类成骨细胞转化,碱性磷酸酶表达明显增高,并表达Ⅰ型胶原。体外复合培养10h,成骨细胞即开始于透明质酸钠凝胶中伸展生长,复合培养7d,成骨细胞在凝胶中分化增殖,分泌细胞外基质。结论:适当浓度成骨诱导剂可成功地将兔MSCs向成骨细胞诱导,透明质酸钠是骨组织工程的良好载体。
Objective： To investigate the feasibility of sodium hyaluronate as scaffold material in bone tissue engineering. Methods： The purified bone marrow stromal ceils separated from rabbit were induced into osteoblasts by dexamethasone, β- glycerophophate and vitamin c and co-cultured with sodium hyaluronate in vitro. The cell-meterial complex was observed under a phase microscope and a scanning electronic microscope to evaluate the interaction between cells and sodium hyaluronate. Results： The form of the cells induced by dexamethasone was converted to like osteoblasts, the expression of alkaline phosphatase increased obviously,and the expression of collagen I was observed. Ten hours after co-culture, the osteoblasts adhered to sodium hyaluronate scaffolds. Seven days later, the osteoblasts differentiated and proliferated in sodium hyaluronate. Extracellular matrix was secreted among osteoblasts. Conclusion： Sodium hyaluronate is good scaffold material for in- ducing rabbit bone marrow stromal cells.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2009年第2期152-154,179,共4页
Chinese Journal of Anatomy
基金
辽宁省科技攻关计划资助项目(2004225003-8)
