摘要
根据羊流产衣原体16SrRNA基因保守序列,设计合成一对引物,通过优化PCR反应条件,成功地扩增出预期的523bp片段,而沙眼衣原体、大肠杆菌、金黄色葡萄球菌及健康鸡胚的卵黄囊膜均未扩增出相应的片段。敏感性试验表明,该体系可检测到2pg的衣原体DNA。
A PCR based detection method was developed using a pair of primers designed from a conserved region of the Chlamydia abortus 16S rRNA gene. This method used all optimized PCR conditions to detect the presence of Chlamydia abortus sequence. A 523 bp fragment was amplified from infected sample, but not from Salmonella, E. coli, Staphylococcus aureus and the yolk sac membrane of health chick embryo. The sensitivity was 2 pg to detect the DNA.
出处
《天津农业科学》
CAS
2009年第2期14-16,共3页
Tianjin Agricultural Sciences
基金
天津市农科院院长基金(06005)
