摘要
目的探讨人脐带间充质干细胞(HUCMSCs)在小鼠体内向男性生殖细胞分化的可能性。方法HUC-MSCs纯化培养后,经显微注射方法移植到不育小鼠睾丸曲细精管,对体内移植后的细胞行免疫组化、免疫荧光及激光共聚焦,观察其存活、迁移及男性生殖细胞的特异性标记物表达等情况。结果HUCMSC体内移植后免疫组化显示其在睾丸微环境中可以存活至少120 d并发生从管腔到基底膜的迁移;免疫组化、免疫荧光、激光共聚焦证明HUCMSCs移植后在睾丸微环境中可以进一步分化,表达生殖细胞特异性标记物。结论体内外定向诱导HUC-MSCs可能可以向男性生殖细胞方向分化,HUCMSCs将可能为治疗男性不育提供一种新的细胞来源,提供一条新的治疗途径。
Objective To study the possibility of differentiation of human umbilical cord mesenchymal stem cells （HUCMSCs） into human male germ cells, and to explore a new source of cells for the treatement of male infertility. Methods HUCMSCs were transplanted into the seminiferous tubules of the testis of infertile mice by microinjection. The survival rate, migration and germ cell markers of HUCMSCs in the mice testis were detected via immunohistochemistry, immuno- fluorescence and confocal laser scanning. Results HUCMSCs can survive in the mice testis for at least 120 days, and they can migrate from the lumens to the basement membrane. Immunofluorescence showed that HUCMSCs can further differentiate in the mice testicular environment, and express the germ cell marker. Conclusions HUCMSCs can survive, migrate and differentiate into early male germ cell-like cells in the infertility mice testis after transplantation.
出处
《中国输血杂志》
CAS
CSCD
北大核心
2009年第3期184-188,共5页
Chinese Journal of Blood Transfusion
基金
2006年广东省科技厅社会发展计划资助课题(编号2006B35901013)
2007年国家自然科学基金资助课题(编号30772354)
2007年广东省科技厅社会发展计划资助课题(编号2007B080701022)
