摘要
目的:建立美味猕猴桃根与茎中熊果酸的含量测定方法。方法:采用HPLC法,色谱柱为Thermo Hypersil BDS C18柱(4.6mm×250mm,5μm);流动相为甲醇-0.2%醋酸铵(85:15);流速为0.8ml/min;柱温为25℃;检测波长为210nm。结果:熊果酸在0.204～2.04μg范围内,峰面积与进样量呈良好的线性关系,r=0.9998;平均加样回收率为101.88%,RSD为1.90%(n=6)。结论:该方法简便可行,重复性好,结果准确,可作为美味猕猴桃中熊果酸的含量测定方法。
Objective： To establish a determination method for the ursolic acid in the stems and toots of Acitinidia deliciosa C. F. liang et A. R. Ferguson. Methods： The way of HPLC was used. Chromatographic column was Thermo Hypersil BDS Cls （4.6mm×250mm,5μm） and the mobile phase was a mixture of methanol-0.2% ammonium acetate （85： 15） with the flow rate at 0.8ml·min^-1. Column temperature was at 25℃and the detector was 210nm. Results： When ursolic acid was in the rang of 2.91-29.1μg, the natural logarithm （Y） of peak area and the natural logarithm （X） of sample size showed a satisfactory linear relationship r= 0.9998;the average recovery of application of sample was 101.88% and RSD was 1.90% （n = 6）. Conclusion： The method is easy which has a good repeatability and an accurate result. It can be the method for the determination for ursolic acid in Fortune euonymus stem.
出处
《广西中医药》
2009年第2期59-61,共3页
Guangxi Journal of Traditional Chinese Medicine
基金
国家自然科学基金地区联合资助项目(NO.30160095)
广西教育厅科研项目(200810LX107)
广西高校人才小高地建设创新团队资助计划
关键词
美味猕猴桃
熊果酸
HPLC
Acitinidia deliciosa C. F. liang et A. R. Ferguson
Ursolic Acid
RP-HPLC.
