摘要
目的从炎症角度探讨冷浴和喷膜疗法联合应用对大鼠早期烫伤创面的影响。方法选择Wistar大鼠75只,随机分为5组,假致伤组、单纯烫伤组、冷浴组、喷膜组及冷浴喷膜组。伤后观察创面的大体情况,分别取不同时相点的创面标本,用干湿重法测定组织含水量;RIA法检测组织中PGE2的含量。结果研究发现冷浴喷膜疗法能显著减轻创面肿胀,减少创面渗出;在各时相点冷浴组、喷膜组及冷浴喷膜组创面组织含水量和PGE2含量均低于单纯烫伤组(P<0.01),但高于假致伤组(P<0.01);在3个处理组中,冷浴喷膜组上述指标最低,存在统计学差异(P<0.05);单纯烫伤组、冷浴组、喷膜组及冷浴喷膜组创面组织含水量与其PGE2含量存在正相关关系(r=0.739,P<0.001)。结论在浅Ⅱ°烫伤创面局部早期应用冷浴喷膜疗法能减轻创面组织水肿,可能与其抑制局部组织炎症反应存在一定关系。
Objective To investigate the anti-inflammatory effect of early local treatment with cooling and spray film on scald burn injury in rats. Methods Seventy-five Wistar rats were randomly divided into 5 groups including the sham-scalded group, untreated scald group, cooling group, spray film group, and cooling plus spray film group with corresponding treatments. After gross observation of the wounds, the tissues at the wounds were sampled at different time points after the injury to determine the total water content （wet： dry weight ratio） and prostaglandin E2 （PGE2） levels using radioimmunoassay （RIA）. Results Treatment with cooling and spray film significantly alleviated the swelling and effusion of the scald bums. At each of the time points, the water content and PGE2 levels in the cooling group, spray film group and cooling plus spray film group were all lower than those in untreated scald group （P〈0.01）, but all higher than those in the sham-scalded group （P〈0.01）. The water content and PGE2 levels were the lowest in cooling plus spray film group, and a significant correlation was noted between the water content and PGE2 levels in the untreated scald group, cooling group, spray film group and cooling plus spray film group （P〈0.01）. Conclusion Local treatment with cooling and spray film can alleviate the edema of superficial Ⅱ degree scald burns in rats probably by reducing the levels of the inflammatory cytokines in the local tissues.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2009年第4期804-806,共3页
Journal of Southern Medical University
关键词
烧伤
冷浴
喷膜
炎症
burns
cooling
spray film
inflammation
